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Cx43 fills the gap between miR-424 and heart hypertrophy: a novel mechanism underlying pulmonary hypertension
Portugal (PorMSIC) - University of Coimbra, Coimbra
Group of Ubiquitin-Dependent Proteolysis and Intercellular Communication, IBILI
English or Portuguese
Type of Research Project
- Basic science
What is the background of the project?
The hallmark of pulmonary arterial hypertension is the inappropriate proliferation of the intima and media layers if the pulmonary arterioles, causing sustained elevation of pulmonary vascular resistance that leads to right ventricle hypertrophy, and subsequently to heart failure and death. The first evidence implying Smurf1 in cardiac hypertrophy was obtained in a mice model of PAH using pulmonary artery constriction. Smurf1 is an ubiquitin ligase that belongs to the Nedd4 family, and it has been shown that Smurf1 is significantly upregulated in pulmonary arteries of monocrotaline-induced pulmonary arterial hypertension rat models. Unsurprisingly, mechanisms that lead to impairment of intercellular communication, normally through increased degradation of Cx43, protein involved in the intercellular communication between cardiomyocytes, have been implicated in various heart diseases, including pulmonary arterial hypertension. Aim of the project: In this project we aim to demonstrate that Smurf1 is a novel regulatory player in Cx43 life-cycle and a crucial player in PAH mechanisms. Techniques and methods: this project will make use of several cellular and molecular biology techniques including: Mutation of plasmid DNA, cell culture and transfection, immunoprecipitation, western blot, immunofluorescence and electron transmission microscopy.
What is the aim of the project?
In this project we aim to demonstrate that Smurf1 is a novel regulatory player in Cx43 life-cycle and a crucial player in PAH mechanisms.
What techniques and methods are used?
This project will make use of several cellular and molecular biology techniques including: Mutation of plasmid DNA, cell culture and transfection, immunoprecipitation, western blot, immunofluorescence and electron transmission microscopy.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is expected to learn some of the techniques employed in our lab and carry out some simple experiments.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
There is not any requirements or legal limitations.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
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