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Genetic association of the genes TLR, NOD2, PARK2, MRC1, MBL, NRAMP1, MIC, VDR with cytokines of leprosy patology
Universidade Estadual de Maringa (UEM)
Universidade Estadual de Maringá, Departamento de Ciências Básicas da Saúde, Av. Colombo, 5790, Bloco T-20, sala 109, Maringá-PR,
Aurea Regina Telles Pupulin
Jeane Eliete Laguila Visentainer
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Leprosy is an immunopathology caused by intracellular organism Mycobacterium leprae. Although there is free public treatment, the disease is still considered a public health problem in many countries. An innate immune response early in infection can be the first line of defence against M. leprae, and it is considered crucial for the development of responses against bacillus step, as essential components has to combat the pathogen effectors, capable of directing adaptive immunity. Certain polymorphisms in genes of the innate response increase the development of the disease and its clinical forms, while other individuals without the same mutation also developed it. The aim is to understand the activation pathways of each receiver and realize where the failure of the immune response which allows the infection and less adequate responses to the outcome of the disease.The present study investigates single nucleotide polymorphisms (SNPs) in genes of Toll- like, NOD2, PARK2, MRC1, MBL, NRAMP1, MIC, VDR and in cytokine regulator genes in the Brazilian population who developed leprosy and compare to a group of healthy and household.
What is the aim of the project?
The present study investigates single nucleotide polymorphisms (SNPs) in genes of Toll-like, NOD2, PARK2, MRC1, MBL, NRAMP1, MIC, VDR and in cytokine regulator genes in the Brazilian population who developed leprosy and compare to a group of healthy and household’s contacts.
What techniques and methods are used?
Genomic DNA will be isolated from 5 mL blood collected in tubes with EDTA by salting-out method, with modification. Genotyping will be performed using PCR-SSP (polymerase chain reaction-sequence specific-primers) and PCR-RFLP (polymerase chain reaction-restriction fragment length polymerase).
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Experiences in the community attended by Sistema Único de Saúde (Health System), in Brazil and in laboratorial practices that help in researches about disease mechanisms.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student should have taken the course of Immunology in his/her graduation.
Modlin RL: The innate immune response in leprosy. Curr Opin Immunol 2010;22:48-54.
Bochud PY, Sinsimer D, Aderem A, Siddiqui MR, Saunderson P, Britton S, Abraham I, Tadesse Argaw A, Janer M, Hawn TR, Kaplan G: Polymorphisms in Toll-like receptor 4 (TLR4) are associated with protection against leprosy. Eur J Clin Microbiol Infect Dis 2009;28:1055-1065.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Modlin RL: The innate immune response in leprosy. Curr Opin Immunol 2010;22:48-54.
- Bochud PY; Sinsimer D; Aderem A; Siddiqui MR; Saunderson P; Britton S; Abraham I; Tadesse Argaw A; Janer M; Hawn TR; Kaplan G: Polymorphisms in Toll-like receptor 4 (TLR4) are associated with protection against leprosy. Eur J Clin Microbiol Infect Dis 2009;28:1055-1065.
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