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Application of polypeptide multilayer films for osteogenic differentiation of human mesenchymal stem cells
Taiwan (FMS-Taiwan) - National Yang Ming University, Taipei
Department of Biomedical Engineering
Professor Woei-Chyn Chu
English (Required) / Mandarin(Accepted)
Type of Research Project
- Basic science
What is the background of the project?
The goal of this study is to fabricate EDC/NHS cross-linked polypeptide multilayer films on coverglass or multi-well plates to effectively investigate the effects of number of layers and concentration of cross-linkers on the osteogenic differentiation of human mesenchymal stem cells derived from umbilical cords.
What is the aim of the project?
This study is aim to investigate the role of substrate stiffness on the proliferation, migration, and cardiomyocyte differentiation of human mesenchymal stem cells (hMSCs).
What techniques and methods are used?
hMSC isolation and cultivation; preparation of protein-coated polyacrylamide substrates with different rigidities ranging from 1 to 30 kPa; traction force microscopy; Immunofluorescence microscopy; cell proliferation and migration assays; Western blot, RT-PCR. -Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)? Preliminary reading materials will be provided before students start working on the project. Training of experimental skills will be provided as well.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will actively participate in meaningful ways, by working on experiments, data analysis, and research presentation. The student needs to be able to learn from a variety of sources, consider multiple possible solutions, and weigh them to discern the best answer.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary reading materials will be provided before students start working on the project. Training of experimental skills will be provided aswell.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Student who has taken cell biology course can basically participate in this project; no special knowledge or prior experimental skill is required. There is no legal limitations in the student’s involvement in the project.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
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