Projects
Name
Analysis of the immunomodulatory properties of scavenger-like lymphocyte receptors
University
Catalonia (AECS) - University of Barcelona (UB) - Hospital Clinic, Barcelona
Domain
Immunology
Departement
Immune receptors of the Innate and Adaptive System Team, Centre Esther Koplowitz
Head
Dr. Francisco Lozano
Tutor
Dr. Fernando Aranda and Dr. Mario Mart
Languages
English, (Spanish accepted but not mandatory)
Duration
8 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes Yes Yes Yes Yes Yes Yes No Yes Yes Yes Yes
Type of Research Project
- Basic science
What is the background of the project?
The lymphocyte surface receptors CD5 and CD6 are highly homologous members of the Scavenger Receptor Cysteine-Rich (SRCR) superfamily expressed by all T lymphocytes and a subpopulation of B lymphocytes (B1a cells). Both receptors are found physically associated to the antigen-specific receptor of T (TCR) and B (BCR) cells, and are able to generate intracellular signals that modulate (either positively or negatively) lymphocyte differentiation and activation proceses, through binding to their endogenous ligands expressed on the surface of antigen presenting cells. CD5 and CD6 should therefore be considered as potential targets for new immunomodulating therapies. What is the aim of the project? The present project aims to analyze and better understand the immunomodulating role of CD5 and CD6 in the immune response against autologous cells (cancer cells) or microbial agents. What techniques and methods will be used? Different experimental models of infection, cancer or autoimmune pathology will be induced in CD5 or CD6 deficient (knock-out) mice, as well as transgenic mice expressing soluble forms of CD5 and CD6 developed by our research group. The effects of the infusion of recombinant soluble forms of these proteins as well as of agonistic/antagonist monoclonal antibodies to conserved epitopes CD5 and CD6 will also be analyzed in wild-type mice. In this way, we hope to set the bases for future immune-mediated therapies aimed at blocking or synergising intracellular signals mediated by CD5 and/or CD6.
What is the aim of the project?
The present project aims to analyze and better understand the immunomodulating role of CD5 and CD6 in the immune response against autologous cells (cancer cells) or microbial agents.
What techniques and methods are used?
Different experimental models of infection, cancer or autoimmune pathology will be induced in CD5 or CD6 deficient (knock-out) mice, as well as transgenic mice expressing soluble forms of CD5 and CD6 developed by our research group. The effects of the infusion of recombinant soluble forms of these proteins as well as of agonistic/antagonist monoclonal antibodies to conserved epitopes CD5 and CD6 will also be analyzed in wild-type mice. In this way, we hope to set the bases for future immune-mediated therapies aimed at blocking or synergising intracellular signals mediated by CD5 and/or CD6.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is expected to do bench work, learn techniques, interpret results and attend and participate in the seminars and laboratory discussions.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Once a week we discuss internally the results obtained by the members of the group. Once a week there are clinical or experimental seminars at the Immunology Department of the Hospital Clínic of Barcelona to which our group also belongs. The IDIPBAPS and the University also organize periodically lectures by internationally renowned researchers.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student is expected to do bench work, learn techniques, interpret results and attend and participate in the seminars and laboratory discussions.
Are there any legal limitations in the student’s involvement
No
Hours
7
Type of students accepted
This project accepts:
- Medical students
- Students in biomedical fields
Articles
- http://www.idibaps.org/research/team/207/immune-receptors-of-the-innate-and-adaptive- system
- https://www.researchgate.net/publication/277843016_CD5_as_a_Target_for_Immune-Based_Therapies
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5495335/
- http://jem.rupress.org/content/213/8/1387
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503133/
- https://www.ncbi.nlm.nih.gov/m/pubmed/27895015/