Projects
Name
Adaptive responses to intermittent hypobaric hypoxia exposure
University
Catalonia (AECS) - University of Barcelona (UB) - Sciences Faculty, Barcelona
Domain
Physiology
Departement
Department of Physiology and immunology, School of Biology, University of Barcelona
Head
Prof. Gines Viscor
Tutor
Prof. G. Viscor and Dr. Joan Ramon Torrella
Languages
English, (Spanish accepted but not mandatory)
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes Yes Yes Yes Yes Yes Yes No Yes Yes Yes Yes
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Our group focuses on the study of adaptive responses caused by the restriction of oxygen supply to tissues. This may be due to environmental causes (altitude) or of systemic origin, which can be considered as normal (exercise) or pathological (e.g. sleep apnea, etc). Our recent work focuses on the responses induced by intermittent exposure to hypobaric hypoxia (simulated altitude). Our focus of interest include: exercise physiology at high altitude, blood rheology and microcirculation in skeletal muscle capillarization, morphometry and metabolic characterization of muscle fibers. We have designed protocols of intermittent exposure to hypobaric hypoxia with application in sports medicine, high altitude expeditions and geographical shift-work.
What is the aim of the project? We are trying to establish the possible efficiency of intermittent hypobaric hypoxia exposure in the recovery of induced muscle damage in rats. We induce muscle damage in rat posterior limbs by means of downhill forced running according to well-known and accepted protocols. Then we consider three groups of animals, one control and two experimental. Control [CON] animals follow a passive recovery (sedentary). Both experimental groups are submitted to intermittent hypobaric hypoxia exposure (IHHE). The first group [HIP] follows only the IHHE protocol, whilst the second experimental group [EHIP] is submitted to IHHE and, in addition, to an exercise protocol. In each sampling stage a representative number of animals will be sacrificed in order to study the mechanisms that could imply a potential improvement in recovery from muscle damage. The evaluation of muscle damage and its time course evolution is done by means of histochemical analysis and the study of some transcriptional factors and protein markers of muscle damage and recovery.
Used Techniques Hypobaric chamber; fitness testing; tolerance to hypoxia testing; blood and plasma rheology; histochemistry; oxidative stress indicators; Reactive oxygen species (ROS). Scavengers.
What is the aim of the project?
We are trying to establish the possible efficiency of intermittent hypobaric hypoxia exposure in the recovery of induced muscle damage in rats. We induce muscle damage in rat posterior limbs by means of downhill forced running according to well-known and accepted protocols. Then we consider three groups of animals, one control and two experimental. Control [CON] animals follow a passive recovery (sedentary). Both experimental groups are submitted to intermittent hypobaric hypoxia exposure (IHHE). The first group [HIP] follows only the IHHE protocol, whilst the second experimental group [EHIP] is submitted to IHHE and, in addition, to an exercise protocol. In each sampling stage a representative number of animals will be sacrificed in order to study the mechanisms that could imply a potential improvement in recovery from muscle damage. The evaluation of muscle damage and its time course evolution is done by means of histochemical analysis and the study of some transcriptional factors and protein markers of muscle damage and recovery.
What techniques and methods are used?
Hypobaric chamber; fitness testing; tolerance to hypoxia testing; blood and plasma rheology; histochemistry; oxidative stress indicators; Reactive oxygen species (ROS) scavengers.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
To collaborate in animal training and in the sessions of muscle damage induction. To participate in muscle and other tissue sampling sessions To participate in muscle processing with histochemistry and other above mentioned techniques. To analyze data derived from the application of these techniques.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
- The student will prepare a scientific report
- The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Knowledge on anatomy and physiology of muscle function, Histological and biochemical, Laboratory techniques Optic microscopy and image analysis Basic levels of biochemistry, cellular biology and statistics Basic skills on laboratory management
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Students in biomedical fields
Articles
- http://www.ncbi.nlm.nih.gov/pubmed/19775218
- http://www.ncbi.nlm.nih.gov/pubmed/19308875
- http://www.ncbi.nlm.nih.gov/pubmed/18270729
- http://www.ncbi.nlm.nih.gov/pubmed/18081508
- http://www.ncbi.nlm.nih.gov/pubmed/10971039
- http://www.ncbi.nlm.nih.gov/pubmed/19874615