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Insulin resistance and regulation of innate immune responses
Greece (HelMSIC) - University of Crete, Heraklion
Department of Clinical Chemistry, Medical School, University of Crete, Voutes, Heraklion
Eleftheria Ieronymaki, Konstantina Lyroni
Type of Research Project
- Basic science
What is the background of the project?
Type 2 diabetes and insulin resistance result in sustained inflammation and predisposition to inflammatory diseases. Macrophages are main mediators of inflammation and are affected by insulin. Evidence from our laboratory has shown that deletion of Akt2, a kinase mediating insulin signals, or IGF1 receptor, the recptor for IGF which is also utilized by insulin, results in insulin resistance in all tissues. Macrophages from this mice have insulin resistance and exhibit an M2-like phenotype, being anti-inflammatory.
Aim of the project is to analise the role of macrophages in insulin resistance-related inflammatory diseases and delineate how insulin resistance affects macrophage function and intracellular signaling. Acoordingly, cells isolated from insulin resistant mice (either the aforementioned knockout mice or mice fed with high fat diet) are analyzed for their activation phenotype and their responses to pro-inflammatory stimuli such as bacterial LPS.
Cell culture models of macrophages either using cell lines or primary macrophages isolated from wild type and different genetically altered mice (Akt2-/-, IGF1R-/- or Adiponectin-/- mice) will be utilized. Gene expression will be detected by PCR and western blot.
What is the aim of the project?
What techniques and methods are used?
What is the role of the student?
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
What are the tasks expected to be accomplished by the student?
Assist in the Lab Work of the project
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Knowledge of immunology and molecular biology. No particular skills are required.
Participation in group meetings and journal clubs with presentation of recent original papers in the field will be available.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- J Immunol. 2014 Jan 1;192(1):394-406. Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9517-22.Immunity. 2009 Aug 21;31(2):220-31
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