Laboratory analysis of trimethylaminuria: from metabolite assay to molecular diagnosis
Taiwan (FMS-Taiwan) - China Medical University, Post-Baccalaureate Chinese Medicine, Taichung
Biophysics and Medical Physics
China Medical University, School of Post Baccalaureate Chinese Medical
Tung-Ti Chang
Wei-De Lin
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes Yes No No No
Type of Research Project
- Basic science
What is the background of the project?
Trimethylaminuria is a disorder in which the volatile, fish-smelling compound, trimethylamine (TMA) accumulates and is excreted in the urine, but is also found in the sweat and breath of these patients. Because many patients have associated body odours or halitosis, trimethylaminuria sufferers can meet serious difficulties in a social context, leading to other problems such as isolation and depression.
What is the aim of the project?
We intend to establish a rapid analysis platform (HPLC-MS/MS) and genetic analysis method that provides patients in need of this service.
What techniques and methods are used?
The techniques and methods applied in this study will include: PCR, DNA sequencing, mass spectrometry.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will follow PI and senior research assistant to learn how to use the laboratory equipment, design the molecular diagnostics methods and perform those experiments by himself/herself.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No, but we can discuss question or problem any time during work time
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The most of basic research skills are required, however, you are encouraged to learn from us. There is no legal limitations in this project.
For the use of students considering participating in the project, further information can be found from the following articles (please add specific articles):
Most of the background knowledge can be obtained from biochemistry and analytic chemistry text book. Here are some references which students can read before coming:
1. Shephard EA, Treacy EP, Phillips IR. Clinical utility gene card for: Trimethylaminuria - update 2014. Eur J Hum Genet. 2014 Oct 22. doi: 10.1038/ejhg.2014.226.
2. Messenger J, Clark S, Massick S, Bechtel M. A review of trimethylaminuria: (fish odor syndrome). J Clin Aesthet Dermatol. 2013 Nov;6(11):45-8.
3. Wooding KM, Auchus RJ. Mass spectrometry theory and application to adrenal diseases. Mol Cell Endocrinol. 2013 May 22;371(1-2):201-7.
4. Edmond de Hoffmann, Vincent Stroobant. Mass Spectrometry: Principles and Applications, 3rd Edition. 2007. John Wiley & Sons, Inc.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- 2014 (Oct); Eur J Hum Genet (10):1038:{Clinical utility gene card for: Trimethylaminuria}
- 2013 (Nov); J Clin Aesthet Dermatol 6(11):45-8:{A review of trimethylaminuria: (fish odor syndrome)}
- 2013 (May);Mol Cell Endocrinol 371(1-2):201-7:{Mass spectrometry theory and application to adrenal diseases}