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PHENOTYPIC DETECTION OF CARBAPENMASES AMOG MULTI-DRUG RESISTANT K. PNEUMONIAE ISOLATES
Greece (HelMSIC) - Aristotle University of Thessaloniki, Thessaloniki
AHEPA UNIVERSITY HOSPITAL, S. KIRIAKIDI 1, 54636 THESSALONIKI, GREECE
SKOURA LEMONIA, ASSISTANT PROFESSOR OF MICORBIOLOGY, ARISTOTLE UNIVERSITY OF THESSALONIKI
1. PROTONOTARIOU EFTHIMIA, LECTURER OF MICROBIOLOGY, ARISTOTLE UNIV. OF THESSALONIKI 2.
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Aquired resistance to b-lactams (mainly carbapenems) among Gram negative pathogens especially Klebsiella pneumoniae and Pseudomonas aeruginosa in hospitals is a growing problem worldwide limiting the clinical utility of several antimicrobials including carbapenems. Of carbapenemases, two major types have been reported up to now, the molecular class B metallo-β-lactamases (MBLs) and the molecular class A K. pneumoniae carbapenemases (KPCs) Accurate detection of these pathogens is necessary to control and prevent their nosocomial transmission. The aim of the present project is to determine the spread of different b-lactamase especially extended spectrum and carbapenemases among b-lactamase-nonsusceptible Gram negative pathogens who are isolated from clinical isolates in AHEPA University Hospital, Thessaloniki, Greece.
All clinical isolates found resistant to b-lactams of 3rd and fourth generation of cephalosporins and/or carbapenems will be phenotypically screened for ESBL and carbapenemase (MBL, KPC, MBL/KPC) production with a combined disk test using meropenem disks without and with EDTA, phenyl-boronic acid (PBA) or both inhibitors. Additionally, in order to detect extended-spectrum-β-lactamases (ESBLs) among cabapenemase-producing strains, the modified CLSI ESBL confirmatory test will be performed using cefotaxime and ceftazidime disks Where necessary, Hodge test will also be applied.
What is the aim of the project?
What techniques and methods are used?
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Assist in the Lab Work of the project
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student will prepare an abstract - No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Some experience in basic laboratory techniques (use of micropipettes, bacteriological loops) would be well-come. No legal limitations. There will be theoretical and practical teaching (lectures and by the bench)
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- Bush K. Jacoby GA. Medeiros AA (June 1995). A functional classification scheme for beta-lactamases and its correlation with molecular structure". Antimicrob. Agents Chemother. 39 (6):1211?33
- Nordmann P. Naas T. Poirel L. (2011). Global spread of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis 17:1791-1798
- Queenan A. Bush K. Carbapenemases: the versatile β-Lactamases. Clin? Microbiol Reviews 2007; 20: 440-458
- Nordmann P. Cuzon G. Naas T. The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria. Lancet Infect Dis 2009; 9: 228-236
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