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Evaluation of photodynamic treatment on cancerous cell line by modern microscopy techniques
Czech Republic (IFMSA CZ) - Palacky University, Olomouc
Biophysics and Medical Physics
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hnevotinska 3, 775 15 Olomouc
prof. RNDr. Hana Kolarova,CSc.
Mgr. Lukáš Malina
Type of Research Project
- Basic science
What is the background of the project?
Photodynamic treatment (PDT) is considered as a promising modality of cancer treatment. PDT uses nontoxic light-sensitive compounds that are exposed selectively to light, whereupon they become toxic to targeted malignant and other diseased cells. Atomic force microscopy (AFM) belongs to modern microscopic techniques with large potential in biological sciences. AFM brings images of surface with high spatial resolution along with probing mechanical properties in such resolution. Mechanical properties belong to the main properties of the cells and tissues of a living organism. They are primarily determined by their cytoskeleton and its physical interaction with the environment.
What is the aim of the project?
Aim of the project is to identify cellular changes after photodynamic treatment in structure and function.
What techniques and methods are used?
Cell culture, atomic force microscopy, fluorescence microscopy, MTT assay.
What is the role of the student?
- The student will mainly observe
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be a member of a research team and it is expected that he(she) will participate in the experimental work. The student will learn a new modality of cancer treatment and how to evaluate cellular changes using modern microscopic techniques.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
For the use of students considering participating in the project, further information can be found from the provided article.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Initial skills required include: preparation and maintence of sterile solutions, working in sterile rooms. It is helpful to have experience with microscopy and/or digital photography, certain knowledge of cell morphology is also necessary. There are no legal limitations in the student's involvement.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Kolar P. et al. The effect of photodynamic treatment on the morphological and mechanical properties of the HeLa cell line; General Physiology and Biophysics (2013) 32; 337-346; doi: 10.4149/gpb_2013042
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