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The role of NMDA receptors in the cardiovascular system: significance of gasotransmitters (NO, CO and H2S) and oxidative stress
Serbia (IFMSA-Serbia) - University of Kragujevac, Kragujevac
Departement of Physiology
Prof. Dr. Gvozden Rosic
Prof. Dr. Vladimir Jakovljevic
Type of Research Project
- Basic science
What is the background of the project?
The N-methyl-D-aspartate receptor (also known as the NMDA receptor or NMDAR), a glutamate receptor, is the predominant molecular device for controlling synaptic plasticity and memory function. Goal of the research is to estimate the potential role of NMDA receptors in regulation of cardiovascular homeostasis, and it`s interactions with gas transmitters and reactive oxygen species. This study includes variety of common neurophysiologic methods and techniques. In this case, lab work will be based on detection of cardiovascular stress, atherosclerosis, NO, H2S impact on CV system and NMDAR modulation.
What is the aim of the project?
To estimate the potential role of NMDA receptors in regulation of cardiovascular homeostasis, and it`s interactions with gasotransmitters and reactive oxygen species
What techniques and methods are used?
1. Langendorff model of retrogradely perfused isloated rat heart The Langendorff heart pre- paration involves the cannulation of the aorta. The he art, onceremoved from a terminally anaesthetized animal, is held withforceps by the opened aortic root and is slipped over a fixedcannula on the perfusion apparatus. This cannula is attached tothe outflow of a reservoir containing an oxygenated perfusionsolution maintained at 37 °C and continuously gassed with a gasmixture containing 5% CO2 and the balance being oxygen for normal aerobic perfusion studies. 2. Biochemical analisys (spectrophotometrical method): Determination of pro-oxidants (TBARS, O 2 - , H 2 O 2 ) Determination of anti-oxidants (SOD, CAT, GSH) 3. Determination of gasotrasnmitters ((NO, CO and H 2 S)) using gas chromatography Principle of gas chromatography: The sample solution injected into the instrument enters a gas stream which transports the sample into a separation tube known as the "column." The various components are separated inside the column. The detector measures the quantity of the components that exit the column. To measure a sample with an unknown concentration, a standard sample with known concentration is injected into the instrument. The standard sample peak retention time (appearance time) and area are compared to the test sample to calculate the concentration.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
To conduct experimental procedures, make statistic calculations, write seminars. The student will acquire basic laboratory and computer skills
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, there will be preliminary reading and lesson.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No special skills are required. There are no legal limitations in the student`s involvement in the project.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
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