[Merida] Insecticide resistance in populations of the southern house mosquito Culexquinquefasciatus (Say, 1823) from Yucatan, Mexico
Universidad Autonoma de Yucatan
Infectious Diseases
Laboratorio de Arbovirología.Centro de Investigaciones Regionales Dr. Hideyo Nogu
Dr. Julian Everardo Garcia Rejon
Dra. Guadalupe del Carmen Reyes-Solis
English, Spanish
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No No No No No No No
Type of Research Project
- Basic science
What is the background of the project?
Culex quinquefasciatus(Say, 1823),the southern house mosquito, is a predominant mosquito species and one of the major domestic pests in urban areas. It is a vector of various arboviruses such as West Nile Virus. There is an estimated global expansion of the transmission of arboviruses, and Cx. quinquefasciatus may play a key role in this expansion because of its high abundance in urban areas. Vector control uses physical methods or chemical or biological insecticides, with the aim of maintaining low levels of insect populations and thus break the cycle of disease transmission and reduce the discomfort caused by insect bites. However, mosquitoes successfully adapt to most insecticides by becoming physiologically or behaviourally resistant to them. Insecticide resistance is a tremendous immense practical problem.
What is the aim of the project?
There are several aims for the project: Aim A: Determine the diagnostic doses of selectedpyrethroids(trans-permethrin, bifenthrin, cypermethrin, deltamethrin, and cyhalothrin) and temephos, as well as the principal biochemical mechanisms responsible forresistance in adult and larvale populations of Cx. quinquefasciatus from Yucatan, Mexico, and the principal biochemical mechanisms responsible for resistance. Aim B: Determine the frequency of the kdr mutation L1014F, which has been clearly demonstrated to be associated with resistance to pyrethroidsand DDT in adult populations of Cx. quinquefasciatus from Yucatan, Mexico
What techniques and methods are used?
DNA extraction, PCR (polymerase chain reaction), Melting Curve, Biossay, Biochemical Assays.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
- The student will be part of a program training to be training in laboratory techniques searching insecticide resistance in vectors. During the research exchange - we expected the student: commitment - willingness to learn.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No special requirements.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Local evolution of pyrethroid pressure offsets gene flow among Aedes aegypti collections in Yucatan State; Mexico. Karla Saavedra-Rodriguez; Meaghan Beaty; Saul Lozano-Fuentes; Steven Denham; Julian Garcia-Rejon; Guadalupe Reyes-Solis; Carlos Machain-Williams; Maria Alba Lorono-Pino; Adriana Flores; Gustavo Ponce; Barry Beaty; Lars Eisen; and William C. Black IV. American Journal of Tropical Medicine and Hygiene. 14(0227). doi: 10.4269/ajtmh.14-0277. ISSN: 1476-1645.
- QTL Mapping of Genome Regions Controlling Larval Temephos Resistance in the mosquito Aedes aegypti. Guadalupe del Carmen Reyes-Solis; Karla Saavedra-Rodriguez; Adriana Flores Suarez; and William C. Black IV. PLOS Neglected Tropical Diseases. 8(10):e3177. doi:10.1371/journal.pntd.0003177. ISSN: 19352727.
- Differential transcription profiles in Aedes aegypti detoxification genes following temephos selection. Saavedra-Rodriguez; Karla; Strode; Clare; Flores; Adriana; Reyes-Solis; Guadalupe; Garcia-Luna; Selene; Hemingway; Janet; Ranson; Hilary; Black; William. Insect Molecular Biology. Volume 23; Issue 2. 199–215 p. April 2014. ISSN: 1365-2583.
- Resistance to permethrin in Aedes aegypti (L.) in Northern Mexico. Adriana E. Flores; Guadalupe Reyes Solis; Ildefonso Fernandez Salas; Francisco J. Sanchez Ramos and Gustavo Ponce Garcia. Southwestern Entomologist. June; 2009. 34(2):167-177. Editorial: Society of Southwestern Entomologists. doi: ISSN: 0147-1724 (print) 2162-2647 (online). Impact factor: 0.407.