Role of NKT cells in the development of life-style diseases in mice; Development and function of cytotoxic T lymphocytes
Japan (IFMSA-Japan) - Kitasato University, Kanagawa(Kanto area)
Department of Immunology, Kitasato University School of Medicine
Kazuya Iwabuchi
Kazuya Iwabuchi, Masashi Sato, Kohji Eshima, Emiko Takeuchi
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes No No No No No
Type of Research Project
- Basic science
What is the background of the project?
NKT cell is literally a subset of T cell that recognizes lipid antigens in the context of CD1d and has a unique role in inflammatory and infections diseases. Eomesodermin is an important transcription factor that is expressed in cytotoxic T lymphocytes.
What is the aim of the project?
To examine the role (mostly accelerating role) of CD1d-restricted NKT cells for development of atherosclerosis and visceral obesity. To examine functions of eomesodermin in develop-ment of cytotoxic T cells. To elucidate regulatory mechanisms for trimming autoreactive B cells by T cells.
What techniques and methods are used?
Techniques to be used are flow cytometry, cell culture, quantification of cytokines by ELISA, immunoblot analysis (Western Blot), PCR and other basic recombinant DNA technologies.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
He or she is expected to learn experimental procedure and scientific reasoning during a research exchange.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Lectures on the subject will be provided on site.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
It is recommended that applicants should have finished basic seiences (especially, biochemistry, physiology, immunolgy microbiology).
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Satoh M et al Phos ONE 6: 28473: doi: 10. 1038/srep 28473
- Satoh M&K Iwabuchi Adipocytes 5(4) :389-393; 2016
- Koji Eshima et al Immunol Lett 144: 7-15; 2012