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[Guanajuato] Evaluation of protein expression of molecules involved in intrauterine growth
Universidad Autonoma de Guanajuato
Department of Medical Sciences Division of Health Sciences, University of Guanajuato, Le&a
Ma. Eugenia Garay Sevilla, PhD.
Gloria Barbosa Sabanero, PhD.
Type of Research Project
- Basic science
What is the background of the project?
Intrauterine growth is a biological process regulated by maternal, placental and fetal endocrine signals. The placenta plays an important role controlling the growth and development of the fetus. Therefore, the study of the expression of these factors in placenta is crucial for understanding the mechanisms that influence birth weight regulation. It is proposed to establish a methodology for the detection of protein expression of molecules involved in intrauterine growth, standardizing this technique in mammalian tissues in which the expression of these molecules be high.
What is the aim of the project?
The aims of the project is to standardize the detection of protein expression of growth factors in control tissues, in order to extrapolate to human placenta technique.
What techniques and methods are used?
1) Measurement of the amount of protein in biological samples (spectrophotometric quantification of protein). 2) Separation of proteins (electrophoresis). 3) Electroblotting of proteins and detection of growth factors with specific antibodies (Western blot).
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Suggested to review in advance the following article.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Abd Ellatife RZ. Size at birth and insulin-like growth factor-I and its binding protein-1 among infants of diabetic mothers. J Matern Fetal Neonatal Med. 2013 Jan;26(1):5-9. doi: 10.3109/14767058.2012.718000. Epub 2012 Sep 20.
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