Monitoring protein-protein interactions with fluorescent probes
Spain (IFMSA-SPAIN)-University of Castilla la Mancha, Albacete
Physiology department, Albacete
Pedro Tranque
MD Juan Llopis and Pedro Tranque PhD
English or Spanish
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Basic science
What is the background of the project?
Over the last two centuries, the discovery and understanding of the principle of fluorescence have provided new means of characterizing physical/biological/chemical processes in a noninvasive manner. Fluorescence approaches offer the potential to sense in real-time extra and intracellular molecular interactions and enzymatic reactions, which constitutes a major advantage over other approaches to the study of biomolecular interactions. To date, with a color palette consisting of fluorescent proteins with different spectra, researchers can "paint" living cells as they desire. This can help us to understand a wide range of biological problems.
What is the aim of the project?
Use non-destructive imaging techniques to quantify interactions between proteins labelled with fluorescent proteins in living cells.
What techniques and methods are used?
Immunohistology, Immunofluorescence, ELISA.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student should be highly interested in Physiology research. He should be helping in the lab and learning and then doing all the necessary techniques to help in the development of the project. The student will acquire practical skills in molecular biological, cell biological and immunologic methods.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
It is required that students have already passed biochemistry, physiology, and histology.
Are there any legal limitations in the student’s involvement
Liability insurance needed
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
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