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Isolation and characterization of mesenchymal stem cells from adipose tissue
University of Pavol Jozef Safarik
Associated Tissue Bank, P. J. Safarik University and L. Pasteur University Hospital
MVDr. Jan Rosocha, PhD.
Ing. Denisa Harvanova, PhD., RNDr. Jana Plsikova, PhD., RNDr. Anna Kopcakova, PhD.
Type of Research Project
- Basic science
What is the background of the project?
Mesenchymal stromal cells (MSCs) represent a key population of cells and are intensively studied nowadays due to their ability to promote hematopoesis, suppress immune response and their proliferative and multilineage differentiation potential. With such properties, MSCs show promise as a possible and good source for research and therapeutic applications. Recently, several studies have been reported of the successful repair of osteochondral defects by the transplantation of cultured chondrocytes. Our aim is to optimize cultivation conditions and characterize mesenchymal stromal cells and chondrocytes.
What is the aim of the project?
The aim of the project is to isolate and characterize chondrocytes and mesenchymal stem cells. This object consists of the following methods: - isolation and cultivation of chondrocytes and mesenchymal stem cells - phenotypic characterization of cell population - in vitro differentiation of cells into three main lineages: osteogenic, chondrogenic and adipogenic - cryopreservation of cultured cells and evaluation of cell viability and quality before therapeutic application
What techniques and methods are used?
? Basic techniques in tissue and cell banking; isolation of chondorytes from articular cartilage, cultivation and phenotypical characterization of mesenchymal stem cells and chondrocytes.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
will to work and participate
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Required skills: basic laboratory practice, working in clean room (Good laboratory practice). There are no legal limitations in the students's involvement in the project.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- May Al-Nbaheen; Radhakrishnanvishnubalaji; Dalia Ali; AmelBouslimi; Fawzi Al-Jassir; Matthias Megges; Alessandro Prigione; James Adjaye; MoustaphaKassem; Abdullah Aldahmash. Human Stromal (Mesenchymal) Stem Cells from Bone Marrow; Adipose Tissue and Skin Exhibit Differences in Molecular Phenotype and Differentiation Potential Stem Cell Rev. 2013 February; 9(1): 32?43
- 2. Patrick C. Baer; Helmut Geiger. Adipose-Derived Mesenchymal Stromal/Stem Cells: Tissue Localization; Characterization; and Heterogeneity. Stem Cells Int. 2012; 2012: 812693. Published online 2012 April 12.
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