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Imaging of glial functions, and their physiological consequences.
Japan (IFMSA-Japan) - Yamanashi University, Yamanashi
Department of Neuropharmacology, University of Yamanashi
Professor, Schuichi Koizumi, PhD.
Yoichi Shinozaki, Eiji Shigetomi
Type of Research Project
- Basic science
What is the background of the project?
Glial cells have pivotal roles in regulation of neuronal and brain functions. However, they have received limited attention. We have worked on glia-to- neuron interaction for over 20 years, and found many of glial functions that are essential for brain functions. We will provide latest information about glial functions to students, and they will experience some important functions of glial cells during their stay in the lab.
What is the aim of the project?
The aim of the project is to image functions of glia (astrocytes) of S1 cortex using 2- photon microscopy, and analyze a causal relationship between glial excitation and neuronal functions in relation to neuropathic pain.
What techniques and methods are used?
This study includes cell cultures, PCR, immunohistochemical analysis, Ca2+ imaging and fluorescent microscopy
What is the role of the student?
What are the tasks expected to be accomplished by the student?
-To learn what glial cells are. -To learn how to manipulate glial cells in vitro. -To learn how to visualize glial functions.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Nothing in particular. But it would be nicer to learn basic knowledge of glia.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Kim et al.; Cortical astrocytes rewire somatosensory cortical circuits for peripheral neuropathic pain. J Clin Inves._126 (5); 1983-1997; 2016.
- Kim et al.; astrocyte-mediated synapse remodeling in the pathological brain; GLIA; in press (DOI:10.1002/glia.23169)
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