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Isolation and characterization of TLQP-21 receptors
Universita degli Studi di Milano-Bicocca
Health Sciences, School of Medicine, University Milano-Bicocca
Prof Grazia Valsecchi
Antonio Torsello, Dr Laura Molteni, Dr Elena Bresciani
English, Spanish, Portuguese
Type of Research Project
- Basic science
What is the background of the project?
TLQP-21, a peptide derived from the VGF precursor, is present in the CNS of rats. We have observed that the central administration of TLQP-21 is effective to block the early development of obesity induced by hyper-caloric diets in mice. Because it is not known the nature of the receptors with which TLQP-21 interacts to induce its metabolic changes we want to identify the receptor/s involved. The identification of the receptor/s that bind TLQP-21 is relevant to understand its cellular mechanisms of action.
What is the aim of the project?
The aim is the characterization of the receptor with which TLQP-21 interacts for studying the mechanisms of action and the structural requirements of the peptide as the necessary premise to the development of new agonist and antagonist compounds that can be used as drugs in clinics to control alimentary related pathologies and in particular dietary obesity.
What techniques and methods are used?
In vitro cell cultures. Real-Time Quantitative PCR Assay (Q-PCR) of mRNAs. Intracellular Ca++ Mobilization Assay by fluorescence emissions from cells loaded with FLUO-4.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will follow the phd student working on this project. During this limited span of time the student will be introduced to cell cultures techniques and Real-Time Quantitative PCR Assay (Q-PCR) of mRNAs. It is expected that the time dedicated to research will be enough to learn and practice these techniques. At the end the student should be able to run in vitro experiments and RNA determinations in complete autonomy.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings and lectures
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Medical students should have completed their basic studies of chemistry and biology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- Biophysical characterization of a binding site for TLQP-21; a naturally occurring peptide which induces resistance to obesity. Cassina V; Torsello A; Tempestini A; Salerno D; Brogioli D; Tamiazzo L; Bresciani E; Martinez J; Fehrentz JA; Verdié P; Omeljaniuk RJ; Possenti R; Rizzi L; Locatelli V; Mantegazza F. Biochim Biophys Acta. 2013 Feb;1828(2):455-60. doi: 10.1016/j.bbamem.2012.10.023. Epub 2012 Oct 30
- Chronic intracerebroventricular injection of TLQP-21 prevents high fat diet induced weight gain in fast weight-gaining mice. Bartolomucci A; Bresciani E; Bulgarelli I; Rigamonti AE; Pascucci T; Levi A; Possenti R; Torsello A; Locatelli V; Muller EE; Moles A. Genes Nutr. 2009 Mar;4(1):49-57. doi: 10.1007/s12263-009-0110-0. Epub 2009 Feb 27
- Ghrelin control of GH secretion and feeding behaviour: the role of the GHS-R1a receptor studied in vivo and in vitro using novel non-peptide ligands. Bresciani E; Tamiazzo L; Torsello A; Bulgarelli I; Rapetti D; Caporali S; Perrissoud D; Moulin A; Fehrentz JA; Martinez J; Locatelli V. Eat Weight Disord. 2008 Sep;13(3):e67-74
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