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Experimental Models for Xenotransplantation Studies: porcine endothelial cells in vitro and genetically modified mouse models
Universita degli Studi di Milano-Bicocca
Laboratory of Animal Models, Department of Surgery and Translational Medicine
Dr. Roberto Giovannoni
Dr. Alessandro Cinti, Dr. Marco De Giorgi, Dr. Claudia Arena, Dr. Elisa Chisci, Dr. Laura
Type of Research Project
- Basic science
What is the background of the project?
The vascular endothelium is the first barrier in a transplantation and xenotransplantation settings. The activation and the injury of the endothelium in xenotransplantation and inflammatory diseases are at the basis of inflammatory and immunological processes leading to organ rejection.
What is the aim of the project?
The project is aimed to investigate the molecular mechanism of cytoprotection induced by expression of a combination of human genes in an in vitro model of porcine endothelial cells exposed to inflammatory stimuli and in vivo in a multi-gene transgenic mouse model.
What techniques and methods are used?
Cell culture; Analysis of DNA of genetically modified mice (PCR); Analysis of RNA expression from cell cultures and animal tissues (RT-PCR and qRT-PCR); Protein expression and signalling (western lot)
What is the role of the student?
- The student will mainly observe
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will understand which are the different stages of the process of translational medicine
The student will participate to the characterization of the molecular mechanism at the basis of cytoprotection
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Pathology, Immunology, Genetics by lectures and face to face teaching. The student will improve his/her technical and experimental skills
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Knowledge of Genetics, Pathology and Immunology is needed. Previous experience (also at basic level) of laboratory general techniques would be useful.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- K.J. Wood; R. Goto; Mechanisms of rejection: current perspectives; Transplantation; 93 (2012) 1?10.
- W. Garrels; Z. Ivics; W.A. Kues; Precision genetic engineering in large mammals; Trends Biotechnol; 30 (2012) 386?393.
- Z. Khalpey; A.H.Y. Yuen; M. Lavitrano; C.G.A. McGregor; K.K. Kalsi; M.H. Yacoub; R.T. Smolenski; Mammalian mismatches in nucleotide metabolism: implications for xenotransplantation; Mol Cell Biochem; 304 (2007) 109?117.
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