Projects
Name
(Concepcion) Functional characterization of Glucose transporters GLUTs in healty and Pathological states
University
Universidad Catolica de la Santisima Concepcion
Domain
Gynaecology/Obstetrics
Departement
Department of Basic Sciences, School of Medicine.
Head
Ximena Ocampo, PhD.
Tutor
Lorena Mardones, PhD.
Languages
English and Spanish
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes No No No No No No Yes No No No Yes
Type of Research Project
- Basic science
What is the background of the project?
GLUTs are multifunctional proteins with different specificity and location. We are interesting in the study of GLUT1 and GLUT8 expression, intracellular location and function in mammary gland during lactation and in pathological states as cancer and mastitis. The specific goal of the project is analyses the expression temporal and spatial of GLUT1 and GLUT8 in mammary gland in the progress of pregnancy and lactation, in different states of mammary carcinomas and different steps in the inflammation of the gland. We are analyzing the expression, the subcellular location, the transcriptional regulation and the kinetic parameters of both transporters in those healthy and pathological states. The project involves several techniques of Biochemistry, Cell Biology and Molecular Cell Biology, as glucose transport assays, protein expression systems, cell culture, immunological techniques, genetic engineering, PCR. The student could use any of those available technologies according to their own interests.
What is the aim of the project?
The specific goal of the project is analyses the expression temporal and spatial of GLUT1 and GLUT8 in mammary gland in the progress of pregnancy and lactation, in different states of mammary carcinomas and different steps in the inflammation of the gland. We are analyzing the expression, the subcellular location, the transcriptional regulation and the kinetic parameters of both transporters in those healthy and pathological states
What techniques and methods are used?
The project involves several techniques of Biochemistry, Cell Biology and Molecular Cell Biology, as glucose transport assays, protein expression systems, cell culture, immunological techniques, genetic engineering, PCR. The student could use any of those available technologies according to their own interests.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The student only will be associated to a small objective, able to be developed in his/her stay into the lab. Is expected to obtain preliminary results or the confirmation of previous results, depend of the theme assigned according to her/his interest and to the project necessities.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
- A white coat is obligatory for this research project. - There are approximately 6 hours of work per day required. - The exchange students participating in this project should already have basic knowledge of chemistry and cell biology. For some specific projects, computer skills are welcome -Use of basic biological equipment (microscopy, micropipette, balance, pH) Concept of Biochemistry, bases of molecular cell biology (PCR, genic expression and regulation)
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Students in biomedical fields
Articles
- -Essential role of intracellular glutathione in controlling ascorbic acid transporter expression and function in rat hepatocytes and hepatoma cells. Free Radic Biol Med. 2012 May 1;52(9):1874-87 (available in: http://www.ncbi.nlm.nih.gov/pubmed/22348976)