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(Concepcion) Immune evaluation of a DNA vaccine against Brucella
Universidad de Concepcion
Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepcion
Helia Bello, Ph.D
Angel Onate, PhD
English and Spanish
Type of Research Project
- Basic science
What is the background of the project?
Brucellosis, caused by Brucella spp., a Gram-negative coccobacillus, is a zoonosis of worldwide occurrence. This disease is associated with high morbidity in animals and humans leading to important economic losses and public health problems in many countries. To prevent bovine brucellosis, immunization with the vaccinal B. abortus RB51 and B. abortus S19 strains has been implemented. Although both vaccines have been effective they cannot eradicate the pathogen and they are pathogenic for humans. It is therefore necessary to develop an effective and safe vaccine able to trigger host immunity against B. abortus. A DNA vaccine that safely expresses immunogens protecting against Brucella infection could be more effective for controlling brucellosis. Perform some immunological techniques necessary to evaluate some immunological parameters. Enzyme-liked immunosorbent assay (ELISA) for antibody and cytokine, Lymphocyte proliferation.
What is the aim of the project?
Perform some immunological techniques necessary to evaluate some immunological parameters.
What techniques and methods are used?
Enzyme-liked immunosorbent assay (ELISA) for antibody and cytokine, Lymphocyte proliferation.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Collaborate with students who are in thesis. How to evaluate immunological parameters in a research where vaccines are developed
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
depends on the student
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
have basic computer skills in a laboratory, basic concepts in immunology; have a basic course in immunology or some related course and basic concepts in Microbiology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- J. BELLO; D. SAEZ; E. ESCALONA; P. VELOZO; C. A. SANTIVIAGO; I. CONTRERAS; A. ONATE. 2016. Mucosal immunization of BALB/c mice with DNA vaccines encoding the SEN1002 and SEN1395 open reading frames of Salmonella enterica serovar Enteritidis induces protective immunity. Epidemiology. Infect. 144: 247-256.
- Riquelme-Neira; R. Rivera; A. Saez; D. Fernandez; P. Osorio; G. del Canto; F. Salazar JC. Vidal; R. Onate; A. 2016. Vaccination with DNA encoding truncated enterohemorrhagic Escherichia coli (EHEC) factor for adherence-1 gene (efa-1’) confers protective immunity to mice infected with E. coli O157:H7. Frontiers in Cellular and Infection Microbiology. 5: Article 104.
- Gomez; L. Alvarez; F. Fernandez; P. Flores; M. Molina; R. Coloma; R. Onate; A. 2016. Immunogenicity and protective response induced by recombinant plasmids based on the BAB1_0267 and BAB1_0270 open reading frames of Brucella abortus 2308 in BALB/c mice. Frontiers in Cellular and Infection Microbiology. 6: Article 117.
- Escalona; E. Saez; D. Onate; A. 2017. Immunogenicity of a multi-epitope DNA vaccine encoding epitopes from Cu-Zn superoxide dismutase and open reading frames of Brucella abortus in mice. Frontiers in Immunology 8: Article 125.
- -Gomez; L.; Llanos; J.; Escalona E.; Saez D.; Alvarez; F.; Molina R.; Flores M.; Onate; A.. 2017. Multivalent fusion DNA vaccines against Brucella abortus. BioMed Researcs International. 2017: 6535479
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