Effect of plasma from obese and insulin resistant (IR) children on endothelial nitric oxide (NO) bioavailability and endothelial Reticulum (ER)-stress
Università degli studi di Chieti
Dept of Clinical and Experimental Sciences University
Prof. Francesco Caciagli
Prof. Assunta Pandolfi, Dr. Natalia Di Pietro
English, Italian
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No No No No Yes No No
Type of Research Project
- Basic science
What is the background of the project?
What is the aim of the project?
To evaluate the potential role of plasma obtained from obese children in regulating vascular function such as no bioavailability. Since ER stress has been recently potentially involved in vascular dysfunction associated to metabolic alterations, we aim to evaluate potential involvement of endothelial ER-stress in obesity.
What techniques and methods are used?
Primary cultures of human umbilical vein endothelial cells (HUVECs) western blot analysis, citofluorimetric evaluations of protein expr. and localiz. confocal microscopy, real time PCR, radiometric evaluation of NO production, ELISA kits to evaluate CGMP levels, nitrotirosine.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
To follow laboratory activities associated to the specific perform in very simple lab tasks. To participate in weekly lab meeting, reporting, weekly activities.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Good basis of scientific knowledge minimum experience in laboratory practice high attitude for team work.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
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