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Clinical characteristics and evaluation of diagnostic tests for Colombian children with congenital toxoplasmosis
Colombia (ASCEMCOL) - Universidad del Quindio, Armenia
Molecular parasitology group
Jorge Enrique Gomez-Marin
Jorge Enrique Gomez-Marin
Requiered: English, Accepted: Spanish
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Congenital toxoplasmosis can result in permanent sequel as blindness or neurological damage in children and it seems to be more severe in South America than in other continents. There is a lack of information about this frequency in Colombia, where no control program is established, although it is a recognized cause of potentially preventable congenital blindness. If sufficient results are obtained during the training period, a poster or oral presentation of the results in Congress or in a structured document possible. The practical skills and knowledge the student will acquire during exchange program: Clinical examination and differential diagnostic approach to infants, children and adults with nodal congenital ocular toxoplasmosis. Basis for analytical interpretation of laboratory tests such as ELISA, PCR, RT-PCR, flow cytometry, cell culture.
What is the aim of the project?
Clinical characterization and therapeutic follow-up of children with congenital toxoplasmosis or patients with ocular toxoplasmosis. Molecular and immunological techniques for diagnosis and follow-up.
What techniques and methods are used?
The methods and tecniques that will be use are the Molecular PCR real time PCR for parasite genes and human, immunological methods for diagnosis, parasite culture, pediatric clinical examination and ophthalmological examination will be performed. Also, the exchange student will have theoretical spaces during his/her exchange through group seminars and journal clubs.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student must actively participate in the tasks that will be assigned in the laboratory and also attend specialized medical consultations of tropical medicine with their tutor. The student will also interact with biologists and Master students for bioinformation and gene search of protozoan parasites as pharmacological targets as well in cell culture methodologies. The student will also participate in our internal seminars where the student will be familiar with the group and their work.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, the student can access spaces such as seminars where you can acquire theoretical knowledge related to all the issues of importance to our project, including: Principles of immunoassay, DNA amplification, cell culture and parasites, western blot: principles of electrophoresis proteins, immunoblot, interpretation in clinical diagnosis, antiparasitic pharmacology (Primetamina sulfa, trimethoprim sulfa, clindamycin, azithromycin). In addition, important scientific articles will be provided so that the student can develop tools and knowledge during their stay in our project.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
In general, students are expected to have skills such as Physical examination, diagnosis and follow-up of children with congenital infection, interpretation of diagnostic tests. However, we do not have requirements such as having passed a specific subject or being in a specific semester. To strengthen all these required skills, always the contact person or the LORE sends the student weeks before the exchange all the articles and previous readings.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- Clinical Validation of a Western Blot Assay for Congenital Toxoplasmosis and Newborn Screening in a Hospital in Armenia (Quindio) Colombia
- A newborn screening programme for congenital toxoplasmosis in the setting of a country with less income
- Positive benefit of postnatal treatment in congenital toxoplasmosis.
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