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[Oaxaca] Expression of O-glycans in MCF-7 cells
Universidad Autonoma de Oaxaca
Biological and Medicine Sciences Research Center of the school of medicine and surgery UAB
Dr. Eduardo Perez Campos
Dr. Pedro Antonio Hernandez Cruz
English and Spanish
Type of Research Project
- Basic science
What is the background of the project?
Breast cancer is a serious threat to the health of women globally and an unrecognized priority in middle-income countries. It is the second cause of death among women aged 30 to 54 and affects all socioeconomic groups. Data on detection, although underreported, show 6000 new cases in 1990 and a projected increase to over 16500 per year by 2020. Genetic, aging, gender and glycosylation changes are risk factors to develop breast cancer. O-glycosylation plays an important role in biological activity of glycoproteins involved in control of cell differentiation. Abnormal O-glycosylation, specially in mucin and mucin type glyproteins, results in exposure of peptide core, as well as in the exposure of the normally cryptic core Tn antigens. Lectins are proteins or glycoproteins that recognize carbohydrates or precipitate glycoconjugates. They are important tools for oligosaccharide characterization as well as isolation of cellular population. Galectin-3, a 31 kDa member of the beta-galactoside-binding proteins, is an intracellular and extracellular lectin which interacts with intracellular glycoproteins, cell surface molecules and extracellular matrix proteins. Galectin-3 is expressed widely in epithelial and immune cells and its expression is correlated with cancer aggressiveness and metastasis. in this work we evaluated O-Glycans and galectina .3 expression in MCF-7 cells by immunohistochemistry and Immunofluorescence, using monoclonal antibody anti galectin-3 and lectins froms Artocarpus integrifolia, Amaranthus leucocarpus and Arachis hypogaea, specific for antigen T
What is the aim of the project?
The aim of this study is to determine, by histochemsitry, flow cytometer the over-expression of antigen TF and galectin-3 in MCF-7 cells using specific lectins for antigen TF and antigalectin-antibody
What techniques and methods are used?
The student will learn techniques about cells culture, to use microscope equipped with fluorescence, analyzes micrographs using AXIOVISON program, RNA extraction and RT-PCR, techniques
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge of Cell Biology, Biochemistry, molecular biology, is required to have previously studied issues concerning breast cancer
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Jimenez M C; Fernandez G; Agundis C; Degand P andZentenoE (2004). Specificityof Amaranthus leucocarpus synhypocondriacus lectin for O-glycopeptides. BiochimBiophys Acta. Nov1;1674(3):282-90
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