Projects
Name
[Tunis]Serological and Molecular identification of mycoplasmas isolated from humans and determination of their antibiotic susceptibility profile
University
Tunisia (ASSOCIA-MED) - University of Tunis El-Manar/Faculty of Medecine of Tunis, Tunis
Domain
Microbiology
Departement
Specialized Mycoplasma Unit, Laboratory of Molecular Microbiology, Vaccinology and Biotechnology Development, Pasteur Institute, Tunis.
Head
Dr. Boutheina Mardessi
Tutor
Dr. Behija Mlik, Dr.Amina ben Allaya, Dr.Elhem Yacoub, Dr.Safa Boujemaa
Languages
English - French
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No Yes Yes Yes No No Yes Yes Yes Yes Yes No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Human mycoplasmas are members of the Mollicutes clan of bacteria. The roles Mycoplasma pneumoniae, mycoplasma Hominis and Ureaplasma spp. in diseases of humans are currently under investigation in Tunisia. M.pneumoniae is a well established pathogens of the respiratory tract. M. hominis and Ureaplasma spp. ,which are common inhabitants of the human genital tract, can induce a wide spectrum of infections of the genitourinary tract in pregnancy and in newborn and they are implicated in infertility.
What is the aim of the project?
-Identification of mycoplasma isolated from humans by culture, serological and molecular assays. -Investigation of their antibiotic susceptibility profiles to antibiotics; in order to demonstrate their implication in respiratory and urogenital pathologies in Tunisian patient
What techniques and methods are used?
-Culture in Mycoplasma Broth -ELISA (enzyme-linked immunosorbent assay) for serological identification -PCR ( Polymerase Chain Reaction ) molecular identification -Study of antibiotic susceptibility by a quantitative broth microdulation technique
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is allowed to observe all the techniques that are described above such as: -Culture in Mycoplasma Broth -ELISA (enzyme-linked immunosorbent assay) for serological identification -PCR ( Polymerase Chain Reaction ) molecular identification -Study of antibiotic susceptibility. Then he is allowed to participate in results’ interpretation and discussion.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
- A white coat is obligatory for this clinical research project.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Students in biomedical fields
Articles
- BMC Infectious Diseases Research article Open Access Genetic variability of the P120' surface protein gene of Mycoplasma hominis isolates recovered from Tunisian patients with uro-genital and infertility disorders Boutheina Ben Abdelmoumen Mardassi*; Hajer Ayari; Awatef Béjaoui-Khiari; Béhija Mlik; Imed Moalla and Faten Amouna Address: Laboratoire des Mycoplasmes; Institut Pasteur de Tunis; 13; Place Pasteur-B.P.74; 1002 Tunis-Belvédère; Tunis; Tunisie Email: Boutheina Ben Abdelmoumen Mardassi* - boutheina.mardassi@pasteur.rns.tn; Hajer Ayari - hajer_a21@yahoo.fr; Awatef Béjaoui- Khiari - awatef.bejaoui@yahoo.fr; Béhija Mlik - behija.mlik@pasteur.rns.tn; Imed Moalla - imedmoalla_ipt@yahoo.fr; Faten Amouna - faten_amouna@yahoo.fr * Corresponding author Published: 5 December 2007 BMC Infectious Diseases 2007; 7:142 doi:10.1186/1471-2334-7-142
- Evidence for the predominance of a single tet(M) gene sequence type in tetracycline-resistant Ureaplasma parvum and Mycoplasma hominis isolates from Tunisian patients Boutheina Ben Abdelmoumen Mardassi; Nadhem Aissani; Imed Moalla; Douaa Dhahri; Abir Dridi and Behija Mlik