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Differentiation of human Induced pluripotent stem(iPS) cells
Japan (IFMSA-Japan) - Shinshu University, Nagano
Department of Histology and Embryology
Professor Katsunori Sasaki MD,PhD
Professor Sasaki,Assistant Professor Fengming Yue, Assistant Professor Daihachiro Tomots
Type of Research Project
- Basic science
What is the background of the project?
Regenerative medicine is surely one of main therapies in the first half of the 21st Century. Pluripotent stem cell biology promotes them actively. In the world Embryonic stem cell(ES cell)- derived cells have been transplanted into about 50 patients including macular degeneration, heart failure, spinal injury, and iPS cell-derived pigment cells into one patient with macula degeneration. This tendency will be prevailing soon. In our project, you learn how to culture human iPS cells, which will give a big chance to engage in advanced medical services to you, after you graduate from medical school.
What is the aim of the project?
The aim of this project is to learn difference of morphogenesis, gene or protein expression between undifferentiated and differentiated stages of iPS cells. Moreover, students will try in this project that they differentiate endodermal progenitor cells expressing PDX-1 from hiPS cells.
What techniques and methods are used?
Cell culture, RT-PCR, immunohistochemistry etc. will be used as analytical tools. there are preliminary readings including related papers, protocols, and lectures as to the method.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Cell handling in addition to RT-PCR and immunohistochemistry Knowledge: Outline of pluripotent stem cells.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes. We have a lecture about Cell handling about human iPS cells.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The practical skills are not necessary. He or she learns them here. The knowledge as to outline of pluripotent stem cells including ES cells and iPScells are necessary, though we have a lecture. Moreover, human iPS cells research does not require any legal limitatons in Japan. In hES cell research, however, there are severe legal limitations. He or she can not do any cell handling of hES cells, but can observe our handling and cultured cells.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- 1.Thomson; JA.; Itskovitz-Eldor; J.; Shapiro; SS.; Waknitz; MA.; Swiergiel; JJ.; Marshall; VS.; Jones; JM.; 1998. Embryonic stem cell lines derived from human blastocysts. Science 282; 1145-1147.
- 2.Takahashi; K.; Tanabe; K.; Ohnuki; M.; Narita; M.; Ichisaka; T.; Tomoda; K.; Yamanaka; S.; 2007. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131; 861-872.
- 3.Johkura K; Cui L; Asanuma K; Okouchi Y; Ogiwara N; Sasaki K.;2004.Cytochemical and ultrastructural characterization of growing colonies of human embryonic stem cells. J Anat. 205:247-255.
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