Enzyme Activity Assay for Lysosomal Storage Disorders using HPLC-FLD and LC-MS/MS
Jordan (IFMSA-Jo), Jordan University of Science and Technology, Irbid
Princess Haya Biotechnology Center / Jordan University of Science and Technology
Dr Laith Eitan, PhD
Amani Haddad
English, Arabic
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
Lysosomal Storage Diseases (LSD) are inherited disorders resulting from a defect in the gene responsible for coding the enzymes that can result in accumulation in the undigested substances which result in an increase in cell size and lead to cell dysfunction and clinical abnormalities, like gaucher, Faby, Kabbe and MPSs.
Techniques: HPLC-FLD and LC-MS/MS
Method: Screening of Enzyme Activity Assay for Lysosomal Storage Disorders approved by centers of disease control and prevention (CDC) ,USA.

Lectures and seminars will be provided.

Student may be Co-authorized in paper with PHBC only.
What is the aim of the project?
Measuring the activity of the enzymes involved in Lysosomal Storage Disorders.
What techniques and methods are used?
Using 1) High performance liquid chromatography with postcolumn fluorescence derivatization. 2) Liquid chromatography–mass spectrometry.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
No specific tasks are expected. Student will receive tasks on a day-to-day basis.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Any requirements listed in EC for IFMSA-Jordan
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Nothing