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Screening for Germline Mutations among Patients Diagnosed with Thalassemia
Jordan (IFMSA-Jo), Jordan University of Science and Technology, Irbid
Princess Haya Biotechnology Center, King Abdullah University Hopital
Shereen Issea, Areej Alquran
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Thalassemia is a blood disorder passed down through families (inherited) in which the body makes an abnormal form of hemoglobin. The disorder results in excessive destruction of red blood cells, which leads to anemia. It is caused by mutation in hemoglobin gene.
What is the aim of the project?
1- To determine the mutation spectrum which cause the disease in Jordanaian population. 2- Learn the molecular technique (PCR, DNA extraction, electrophoresis, PCR sequencing, etc.) 3- How to analyze the electrogram. 4- How to use the bioinformatics tools (NCBI, enesmbl, chromose, primer)
What techniques and methods are used?
We use DNA sequencer method to detect the mutation in the gene by DNA extraction from blood, then PCR using thermalcyclear ,then gel electrophoresis, purification and sequence PCR then cleaning and loaded in 3031xl genetic analyzer. There will be some lectures on the subject.
What is the role of the student?
- The student will mainly observe
What are the tasks expected to be accomplished by the student?
- The student will learn how to use the molecular techniques -PCR - DNA extraction - electrophoresis - etc.. to diagnose the sample.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
- Knowledge of Genetics principles. - Complete all training hours successfully. Any other requirement listed in the EC for IFMSA-Jordan.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
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