Adipose tissue-vessels cross-talk in coronary artery disease. New biomarkers and therapeutic targets
Spain (IFMSA-SPAIN)-University of Santiago de Compostela, Santiago de Compostela
Cardiovascular Area, Health Research Institute of Santiago de Compostela
Jose Ramon Gonzalez Juanatey
Dr. Ezequiel Alvarez Castro, Dr. Sonia Eiras Penas
Required: English. Accepted: Spanish
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes Yes Yes Yes Yes Yes Yes No Yes Yes Yes Yes
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
The main cause of cardiovascular mortality is coronary artery disease and there is no curative treatment. Thus, it is very neccesary to find new therapeutical approaches and targets. In this way, our hypothesis is that epicardial adipose tissue (EAT) plays a crutial role through hormonal interaction over the coronaries. Thus, in the last years, this relationship has been studied and it is known that EAT volume is associated with abdominal fat and seems to be a predictor of coronary atherosclerosis [1]. Moreover, several works have found that this fat is able to express and release several proinflammatory cytokines, peptides related to blood pressure, etc. [2, 3]. In this way, this tissue could play an important role as endocrine or paracrine mode over the coronaries and myocardium for aggravating the atherosclerotic vascular walls or myocardium dysfunction. This mechanism could be explained by the interaction between among the cellular compound of EAT, fibroblasts, endothelial cells, macrophages, lymphocytes, etc
What is the aim of the project?
Study the epicardial fat and coronaries cross-talk for understanding the physiopathological mechanisms of coronary artery disease and providing new therapeutic targets.
What techniques and methods are used?
Primary culture cells: endothelial, adipose and stromal cells isolation from human biopsies. Molecular and cellular characterisation of cells (inmunocytochemistry, mitochondrial activity, glucose metabolism, differentiation assays, etc.). Proteomics and transcriptomics assays (2D or 1D-gels, western blot, real time PCR for mRNA expression, etc.) Data analysis: SPSS, Excell
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Minimal knowledge of lab routine (for example: solution preparation, calculate concentrations
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There will be clinician seminars and lectures in the lab.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Minimal knowledge of lab routine (for example: solution preparation, calculate concentrations
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
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