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Influence of nutricional factors in neuronal survival in the hippocampal formation of the rat
Portugal (PorMSIC) - University of Porto, Porto
Department of Anatomy, Faculty of Medicine of University of Porto
Professor Maria Dulce Madeira
Professor Armando Cardoso
Type of Research Project
- Basic science
What is the background of the project?
The typical diet of western societies,very rich in mono- and dissacharides, probably affects the central nervous system contributing to accelerated cognitive decline and increased risk of neurodegenerative diseases. On the other hand, food restriction protects the central nervous system from ischemic and metabolic lesions and others related to increasing of oxidative stress.
What is the aim of the project?
Clarify important aspects of neurogenesis and apoptosis in the hippocampus induced by ingestion of high-sucrose diet in a Rat model. On the other hand, the study of the same parameters during the food restriction will give some light to the mechanisms involved in the neuroprotection related to this deprivational condition.
What techniques and methods are used?
Experimental study using Rat models. Animal perfusion. Brain sectioning methods. Immunocytochemistry for several neurogenesis and cell proliferation markers, invluding BrdU,DCX, and pHisH3-Ser10. TUNEL immunocytochemistry. Quantification of the neurons immunostained applying unbiased stereological methods.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The students will have an important role in processing the rat brains and will participate in all laboratory tasks. Furthermore students will also quantificate part of the material using several techniques routinely used in our laboratory. What should be the outcome of the student
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Students will be introduced to the investigation topic and laboratory technics used in the study. Students will read some of the most important papers of the topics unuder investigation.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
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