Projects
Name
Viral infections in blood stem cells graft recipients in post-transplantation period.
University
Poland (IFMSA-Poland) - Medical University of Warsaw, Warszawa
Domain
Microbiology
Departement
Chair and Department of Medical Microbiology, Virology Laboratory
Head
Prof. Grazyna Mlynarczyk, PhD
Tutor
Maciej Przybylski, PhD
Languages
English, Russian, Polish
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes No No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Introduction of immunosuppression in patients undergoing HSCT (hematopoietic stem cell transplantations) is the major factor increasing risk of severe viral infections. List of the viruses which can cause dangerous infections in HSCT graft recipients comprise mostly a pathogens widely distributed within a population, usualy causing asymptomatic or subclinical infections in immunocompetent individuals. Opportunistic viral infections in immunosuppressed patients occur mainly in bone marrow ablation and conditioning period (from first to third week after transplantation), and in about 10% of graft recipients, in which chronic neutropenia is an effect of immunosuppresion, period of increased susceptibility to viral infections is longer and may last for 2-3 months after transplantation. Viral infections in HSCT recipients are caused in first place by herpesviruses, next by para- and orthomyxoviruses, parvovirus B19, polyomaviruses and adenoviruses. Of course, special attention should be paid to herpesviruses, because of their ability to establish an latency state after primary infection. Symptomatic viral disease as a result of latent herpesvirus reactivation is more popular event in adult graft recipients population than disease due to primary infection. From other hand, results of primary infections are usualy much more severe. Detection of suspected viral agent in acute infection and routine monitoring of the graft recipients in search for the virus before advent of clinical symptoms are the most important tasks of the virology laboratory in hematology.
What is the aim of the project?
Within project Viral infections in blood stem cells graft recipients in post-transplantation period there are several ongoing and planned sub-projects realized in Virology Laboratory: a. Defining the role of peripheral blood limphocytes in harbouring the latent alphaherpesviruses b. Analysis of viral etiological agents of hemorrhagic cystitis in HSCT recipients. c. Detection of the mutations in UL97 and UL54 genes of cytomegalovirus (CMV), responsible for resistance of virus strains to ganciclovir. d. Role of HHV-6 and HHV-7 in the clinical course ov CMV infection. e. Identification of the host-dependent factors correlated with various forms of EBV infections in HSCT recipients.
What techniques and methods are used?
1. Real-time PCR 2. End-point PCR 3. Sequencing of PCR products using Sanger method 4. Direct immunofluorescence assay (DFA) 5. Cultivation of the viruses in cell cultures 6. Enzyme-linked immunosorbent assay
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Participation and self-development.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Student should have completed microbiology course at university level (medical microbiology is preferred).
Are there any legal limitations in the student’s involvement
No
Hours
5
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
Articles
- Fisher RA. Cytomegalovirus infection and disease in the new era of immunosuppression following solid organ transplantation. Transpl Infect Dis. 2009 Jun;11(3):195-202.
- Cytomegalovirus. Am J Transplant. 2004 Nov;4 Suppl 10:51-8.
- Mori I; Nishiyama Y. Herpes simplex virus and varicella-zoster virus: why do these human alphaherpesviruses behave so differently from one another? Rev Med Virol. 2005 Nov-Dec;15(6):393-406
- Yamashita N; Kimura H; Morishima T. Virological aspects of Epstein-Barr virus infections. Acta Med Okayama. 2005 Dec;59(6):239-46.
- Echavarria M. Adenoviruses in immunocompromised hosts. Clin Microbiol Rev. 2008 Oct;21(4):704-15