Projects
Name
Determine extent of Microbioma of bronchial secretions using molecular methods
University
Catalonia (AECS) - Autonomous University of Barcelona (UAB) - Hospital Parc Tauli, Barcelona
Domain
Pneumology
Departement
Pneumology Department, Hospital Universitari del Parc Tauli de Sabadell
Head
Eduard Monso
Tutor
Eduard Monso
Languages
English, French, Spanish, Portuguese, Italian
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes Yes Yes Yes Yes Yes Yes No Yes Yes Yes Yes
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
The bronchial tree is considered sterile in healthy subjects, but bacteria are often recovered from bronchial secretions in patients with chronic bronchitis during periods of clinical stability. When sputum has been used in chronic bronchitis for the identification of bronchial colonisation, cultures positive for bacteria have been found in one-fifth to three quarters of the patients, in most cases to a single microorganism. Most bacteria recovered from stable chronic bronchitis patients show low microbial loads, with high loads being found almost exclusively when H. influenzae is the colonising bacteria. The mechanisms behind the recovery of low-load bacteria from sputum in patients with chronic bronchitis is open to debate, as it may be related to oropharingeal bacteria that have migrated to the bronchial tree or to flora colonising specifically the lower airway. The use of molecular methods like PCR amplification of the 16S rRNA gene followed by cloning and traditional Sanger sequencing has allowed the identification of many bacterial species previously undetected by culture. In recent years, the application of pyrosequencing to PCR-amplified products from human samples has taken the study of microbial diversity to an unprecedented level of detail. This approach has not been followed in pulmonary tract samples, and it has not been determined the extent the microbial diversity present in oropharingeal and bronchial secretions.
The aim of the present study was to identify airway bacteria unrecognized through culture in patients with chronic bronchitis in the absence of signs and symptoms of infection, as well as to examine the complexity of microbial flora in these patients by the use of amplification and pyrosequencing of the 16S rRNA gene. In addition. We will compare the microbial diversity recovered from, nasal, oropharingeal and bronchial secretions, and exhaled breath condensate samples from the same individuals.
What techniques and methods will be used? Orophageal and nasal sampling, induced sputum, exhaled breath condensate, DNA extraction.
What is the aim of the project?
The aim of the present study was to identify airway bacteria unrecognized through culture in patients with chronic bronchitis in the absence of signs and symptoms of infection, as well as to examine the complexity of microbial flora in these patients by the use of amplification and pyrosequencing of the 16S rRNA gene. In addition. We will compare the microbial diversity recovered from, nasal, oropharingeal and bronchial secretions, and exhaled breath condensate samples from the same individuals.
What techniques and methods are used?
Orophageal and nasal sampling, induced sputum, exhaled breath condensate, DNA extraction.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Obtention of clinical samples of oropharyngeal and bronchial secretions including exhaled breath condensate under supervision, extraction of DNA from samples, database registration of the obtained results.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No, only preliminary readings.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Respiratory phisiopathology and Clinical pneumology. Assistance to patients only done under direct supervision by staff.
Are there any legal limitations in the student’s involvement
No
Hours
7
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
Articles
- http://www.ncbi.nlm.nih.gov/pubmed/15851640
- http://www.ncbi.nlm.nih.gov/pubmed/16478847
- http://www.ncbi.nlm.nih.gov/pubmed/12181400
- http://www.ncbi.nlm.nih.gov/pubmed/10996579
- http://www.ncbi.nlm.nih.gov/pubmed/19643939
- http://www.ncbi.nlm.nih.gov/pubmed/20052417
- http://www.ncbi.nlm.nih.gov/pubmed/21364979