Viral vector based gene delivery to the lungs of asthmatic animals
Taiwan (FMS-Taiwan) - Chang Gung University, Taipei
Department of Microbiology and Immunology, Chang Gung University
Yi-Ywan Margaret Chen
Dr. Ming-Ling Kuo
4 weeks
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Type of Research Project
- Basic science
What is the background of the project?
We will introduce anti-inflammatory genes into the lungs of athmatic mice with AAV viral vectors which will specifically target lung tissues. The gene sequences may direct target inflammatory mediators or siRNA fragments.
What is the aim of the project?
The specific aim is to apply viral based vectors to deliver gene sequences that have therapeutic potential to modulate asthmatic inflammatory responses.
What techniques and methods are used?
Techniques: ELISA, RT-PCR, DNA construction, cell culture, flow cytometry, animal asthma model
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The exchange students have to learn and perform the basic techniques that are interested by them and assist data analyses of the involved project.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The pathogenesis and novel therapeutic approaches of asthma inflammatory responses will be introduced and discussed through the experiments and lab meetings. One or two most recent papers will be presented by graduate students (as seminar) every month. Student will understand pathogenesis of asthma in more details.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
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- 2008; Clin Microbiol Rev (21): 583-593:{}
- 2009; Hum Gene Ther (20):1-10:{}