Projects
Name
(Santiago de Chile) Immunohistochemical localization of teneurin-4 in human cancer cell lines
University
Universidad del Desarrollo
Domain
Genetics
Departement
Human Genetics Center, Clinica Alemana
Head
Gabriela Repetto, MD. PhD.
Tutor
Annemarie Ziegler, Ph.D.
Languages
English, Spanish, German
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No No Yes Yes No No No
Type of Research Project
- Basic science
What is the background of the project?
Recently, we and others have found evidence for a role of neural teneurins in some types of human cancer. The functional contribution as well as the expression patterns of teneurins in human tumors remains unknown. This project will examine the expression patterns and subcellular distribution of teneurin-4 in cultured human cancer cell lines. The aim of the project consists in obtainig a first insight into the expression profile of human teneurin-4 in human cancers, by using cultured tumor cell lines as a model. The techniques and methods that will be used ar cell culture techniques, immunohistochemistry and associated procedures (fixation techniques, handling of antibodies, general laboratory procedures, microscopy, data evaluation). Will be theoretical teaching provided to the student during the exchange as prliminary readings in the form of scientific publications, project discussion incluiding theoretical and practical support; participation in journal-clubs and lab meetings.
What is the aim of the project?
The aim consists in obtaining a first insight into the expression profile of human teneurin-4 in human cancers, by using cultured tumor cell lines as a model.
What techniques and methods are used?
Cell culture techniques; immunohistochemistry and associated procedures (fixation techniques, handling of antibodies, general laboratory procedures, microscopy, data evaluation)._______________________________________________________________
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
He/she will participate in a larger project, where he/she will devote time to perform practical experiments aimed at studying the expression patterns and subcellular localization of teneurin-4 in cultured human cancer cell lines by immunohistochemical techniques.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary readings in the form of scientific publications. Project discussion including theoretical and practical support. Participation in journal-clubs and lab-meetings. ________
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
- The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student needs special knowledge of practical biochemistry course an advantege, or equivalent laboratory-based experience. There are no legal limitations in the students involvement in the project. For the use of students considering participating in the project, further information can be found from the following articles: 1) TR Young, CA: Leamey. Teneurins: Important regulators of neural circuitry. International Journal of Biochemistry & cell biology 41 (2009) 990-993. 2)U Vinatzer, M Gollinger, L Muellauer, M Raderer, A Chott, B Streubel. Mucosa-Associated Lym phoid Tissue Lymphoma: Novel Translocations Incluiding Rearrangements of ODZ2, JMJD2C and CNN3. Spanish, English und German are languages accepted. will be accept to the project medical students, graduated students, pre-medical students and students in biomedical fields.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
- Students in biomedical fields
Articles
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