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(Santiago de Chile) Induction of TH1/TH17 and inhibition of TH3 cell profiles in melanoma patients treated dendritic cells-based immunotherapy
Universidad de Chile
Juan Carlos Aguillon, PhD
Mercedes Lopez, PhD
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
To study the cellular and soluble factors in serum or peripheral blood of melanoma patients immunized with cell therapy based on dendritic cells. The aim of the project is to study the statistical correlation between the percentage of TH17 cells in peripheral blood mononuclear cells (PMBC) from treated patients and serum levels of TGF-beta, IL-10, IL-17A and VEGF obtained from patients in different stages of treatment. The techniques and methods will be used are ELISA, flow citometry, cell culture and statistical analysis. Practical skills and knowledge the student will acquire is to contribute in laboratory techniques, statistical analysis and clinical protocols.
What is the aim of the project?
To study the statistical correlation between the percentage of TH17 cells in peripheral blood mononuclear cells (PMBC) from treated patients and serum levels of TGF-β, IL-10, IL-17A and VEGF obtained from patients in different stages of treatment .
What techniques and methods are used?
ELISA, flow cytometry, cell culture, statistical analysis.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Contribution in laboratory techniques and statistical methods.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Reading of papers and bibliographic seminars.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Cell biology and molecular immunology. No statutory limitation. Cell culture,and clinical protocols.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system - Students in biomedical fields
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