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Role of the chemokine network in mediating dendritic cell? T cell interactions with relevance for HIV-1 pathogenesis
Université de Montréal
Microbiology and Immunology, Universit
Pr Pierre Belhumeur
Dr. Petronela Ancuta
French is welcome but not necessary
Type of Research Project
- Basic science
What is the background of the project?
Our overall goal is to characterize the functional heterogeneity of monocyte-derived dendritic cell (DC) and CD4+T cell subsets in the context of HIV pathogenesis and this by taking advantage of the differential expression of chemokines and chemokine receptors on functionally distinct DC and T cell subsets.
What is the aim of the project?
Our aims are to: (1) characterize the contribution of CD16+ and CD16- monocyte derived DC to antigen presentation and HIV cell-to-cell transmission, (2) identify CD4+ T cell subsets that harbor HIV reservoirs in vivo, and (3) determine the role of chemokines (e.g., ligands of CCR4, CCR6 and CXCR3) in immunity and HIV pathogenesis.
What techniques and methods are used?
We are using several state of the art techniques including multicolor flow cytometry, cell culture, manipulation of HIV-infected samples in BSL3 conditions, cells sorting by MACS and FACS, real-time PCR and PCR, western blotting, ELISA, Affymetrix microarray technology and confocal microscopy.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The role of the student will be to interact efficiently with members of the research group with the aim to acquire new skills in experimental and theoretical immunology/virology. The student is expected to discuss the scientific project and critically interpret results of the laboratory. Students are encouraged to ask new questions and provide suggestions/solutions. Results generated by the student will be included, if valid, in future publications or presentations and his/her name will be listed as a co-author. The student will chose among different projects in the laboratory the topic of interest. The student will learn at least two of the techniques running in the lab (see the list provided above). Theoretically, the student will become familiar with the following concepts: (1) antigen presentation and immunological synapse formation at the interface between dendritic cells and T cells, (2) HIV-1 viral cycle and cell-to-cell transmission, (3) chemokine-mediated costimulation of T cells.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will chose among different projects in the laboratory the topic of interest. The student will learn at least two of the techniques running in the lab (see the list provided above). Theoretically, the student will become familiar with the following concepts: (1) antigen presentation and immunological synapse formation at the interface between dendritic cells and T cells, (2) HIV-1 viral cycle and cell-to-cell transmission, (3) chemokine-mediated costimulation of T cells.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication - No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge in immunology, cell biology and/or molecular biology is required. The student is expected to preserve the confidentiality of laboratory projects and results.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Ancuta P et al. J Immunol. 176; 5760-5771.(2006)
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