Projects
Name
Association study between gene polymorphisms and drug susceptibility in schizophrenia patients
University
Thailand (IFMSA-Thailand) - Faculty of Medicine, Srinakharinwirot University, Bangkok
Domain
Biochemistry
Departement
Biochemistry
Head
Assoc.Prof. Ramima Watanapokasin, PhD
Tutor
Assoc.Prof. Wasana Sukhumsirichart, PhD
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes No No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Schizophrenia is a psychosis that affects about 1% of the population worldwide, with high heritability (80% or more). It is characterized by positive symptoms (delusions, hallucinations, thought disorders),negative symptoms (lack of motivation, alogia, social withdrawal), and cognitive symptoms. Schizophrenia patients exhibit abnormalities in brain structures and chemistry that are considered to be linked with pathogenesis. Vast lines of evidence suggested that schizophrenia is a polygenic disorder in which many genes and environmental factors are involved in the etiology. Twin and family studies suggest that genetic factors play a major role in schizophrenia. A variety of genetic studies reported several chromosomal regions associated with schizophrenia and related phenotypes.
This study aims to investigate association between single nucleotide polymorphisms (SNPs) in dopamine receptor D2 gene and susceptibility to first and second generation psychiatric drug in schizophrenia patient.
What is the aim of the project?
This study aims to investigate association between single nucleotide polymorphisms (SNPs) in dopamine receptor D2 gene and susceptibility to first and second generation psychiatric drug in schizophrenia patient.
What techniques and methods are used?
- DNA extraction using commercial kit - Primer designation by using primer design program - Real-time PCR - High-resolution melting (HRM) analysis - Statistical analysis using chi-square test of SPSS software
What is the role of the student?
What are the tasks expected to be accomplished by the student?
The role of the student is help a researcher perform experiments such as extraction of DNA from whole blood, design primer for PCR, real-time PCR amplification, HRM analysis, and analysis of genotype distribution by using Chi-square test of the SPSS software. In addition, the student can help researcher to search the articles and discussion the results in which a medical basic knowledge is need. Because there are a lot samples, so the student can practice himself/herself to get skill in doing experiment.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Able to use the micropipette (autopipette). Special knowledge needed are the understanding about single nucleotide polymorphisms (SNPs), real-time PCR, and statistic analysis methods.
Are there any legal limitations in the student’s involvement
No
Hours
7
Type of students accepted
This project accepts:
- Medical students
Articles
- Dworkin RH; Lenzenweger MF: Symptoms and the genetics of schizophrenia: implications for diagnosis. Am J Psychiatry 1984; 141:1541?1546.
- Kendler KS; Diehl SR: The genetics of schizophrenia: a current; genetic epidemiologic perspective. Schizophr Bull 1993; 19:261?285.
- Almasy L; Gur RC; Haack K; Cole SA; Calkins ME; Peralta JM; Hare E; Prasad K; Pogue-Geile MF; Nimgaonkar V; Gur RE: A genome screen for quantitative trait loci influencing schizophrenia and neurocognitive phenotypes. Am J Psychiatry 2008; 165:1185?1192.
- Lewis CM; Levinson DF; Wise LH; DeLisi LE; Straub RE; Hovatta I; Williams NM; Schwab SG; Pulver AE; Faraone SV; et al: Genome scan meta-analysis of schizophrenia and bipolar disorder; part II: Schizophrenia. Am J Hum Genet 2003; 73:34?48.
- Panichareon B; Nakayama K; Thurakitwannakarn W; Iwamoto S; Sukhumsirichart W. OPCML gene as a schizophrenia susceptibility in Thai population. 2012 J Mol Neurosci;46(2):373-7.
- Panichareon B; Nakayama K; Iwamoto S; Thurakitwannakarn W; Sukhumsirichart W. Association of CTXN3-SLC12A2 polymorphisms and schizophrenia in a Thai population. Behav Brain Funct. 2012;8(1):27.
- Preliminary reading: Panichareon B; Nakayama K; Thurakitwannakarn W; Iwamoto S; Sukhumsirichart W. OPCML gene as a schizophrenia susceptibility in Thai population. 2012 J Mol Neurosci;46(2):373-7.