Projects
Name
MC/Treg interaction in the development of autoimmune disease
University
Universita degli Studi di Udine
Domain
Immunology
Departement
Biomedical Science and Technology Department
Head
Prof. Pietro Enrico Di Prampero
Tutor
Prof. Carlo Pucillo
Languages
english italian
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes No No No No No
Type of Research Project
- Basic science
What is the background of the project?
MC/Treg interaction in the development of autoimmune disease
What is the aim of the project?
The aims of the project are 1) to address the importance of MC in the modulation of self-reactive T cells in autoimmune diabetes. Expansion and diabetogenic potential of islet-reactive T cells in nonobese diabetic (NOD) mice that lack either MC NOD-KitW/Wv mice or MC/T cell interaction (OX40KO mice) will be analyzed. 2. To evaluate the role of cross-talk between MC and Treg cells for prevention of autoimmune diabetes. We will test the hypothesis that Treg cells counter-regulate diabetogenesis through modulation of MC function. Specifically, Treg-modulated MC could reduce degranulation of pro-inflammatory cytokines and antigen-presenting function upon islet-reactive T cells.
What techniques and methods are used?
The student will use cellular and molecolar techniques direct to investigate the cellular events following th eTreg/mast cell interaction
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will work in pair with the tutor if she/he is able, or he/she will follow the work of the tutor The student will perform basic lab research and it will be introduced to the study of novel therapeutic approaches of autoimmune disease.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Scientific papers will be provided and the student will attend to lab meeting, in English.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
It will be appreciate if the student has basic knowledge of immunology, molecular biology and cellular biology and biochemistry at theoretical level at least. For the use of students considering participating in the project, further information can be found from the following articles: Frossi B, Rivera J, Hirsch E, Pucillo C. Selective activation of Fyn/PI3K and p38 MAPK regulates IL-4 production in BMMCunder nontoxic stress condition. J Immunol. 2007 Feb 15;178(4):2549-55. Frossi B, De Carli M, Pucillo C. The mast cell: an antenna of the microenvironment that directs the immuneresponse.J Leukoc Biol. 2004 Apr;75(4):579-85. Galli SJ, Nakae S, Tsai M. Mast cells in the development of adaptive immune responses.Nat Immunol. 2005 Feb;6(2):135-42.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
Articles
- Frossi B; Rivera J; Hirsch E; Pucillo C. Selective activation of Fyn/PI3K and p38 MAPK regulates IL-4 production in BMMCunder nontoxic stress condition. J Immunol. 2007 Feb 15;178(4):2549-55.
- Frossi B; De Carli M; Pucillo C. The mast cell: an antenna of the microenvironment that directs the immuneresponse.J Leukoc Biol. 2004 Apr;75(4):579-85.
- Galli SJ; Nakae S; Tsai M. Mast cells in the development of adaptive immune responses.Nat Immunol. 2005 Feb;6(2):135-42.