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Molecular biology of the oxidative stress
Universita degli Studi di Udine
Biomedical Science and Technology Department
Prof. Pietro Enrico Di Prampero
Prof. Gianluca Tell
Type of Research Project
- Basic science
What is the background of the project?
Many neurodegenerative disorders appear to derive from oxidative stress. APE1/Ref-1 is an essential protein for the life of a mammalian cell. It has a dual role: it is a key enzyme in the BER pathway of DNA repair and is an activator of the expression of many genes. Its action is especially important under oxidative stress. The aims of the research are 1.the chacterization of the molecular network in which APE1 plays a central role by modern gene expression technologies through microarrays and differential and functional proteomics analysis; 2. the role of the post-translational modifications, in particular sumoylation, in controlling the different APE1 biological functions in particular regard to neuronal cell system.
What is the aim of the project?
By using a conditional gene expression knockdown by RNA interference technology in SHSY5Y neuronal cell line, the molecular changes associated to APE1 loss of expression will be inspected. Both mRNA expression profile and proteomics to investigate the molecular changes associated to APE1 loss-of-expression by siRNA technology will be combined. By this approach the influence of APE1 on neuronal biology will be studied. To dissect direct and indirect effects knock-in experiments with mutant APE1 will be performed. Finally the different interacting partners of APE1 under basal and oxidative-stress conditions will be identified by functional proteomics studies. These data will clarify the molecular reasons for the essentiality of this protein for neuronal cells and will give an understanding of the molecular details responsible for the fine-tuning of APE1, providing the molecular basis for developing new drugs to specifically modulate the different biological functions of this multifunctional protein.
What techniques and methods are used?
The student will use also cellular and molecolar techniques like proteomic technique and gene silentiation.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
It will be appreciated if the student has basic knowledge of molecular biology and biochemistry.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
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