Visualization of the lysosomal enzyme delivery system
Fukushima Medical University
Department of Anatomy and Histology, Fukushima Medical University, 1-Hikarigaoka, Fukushim
Satoshi Waguri, MD, PhD, Professor
Satoshi Waguri, MD, PhD, Professor
English (It is highly recommended to learn Japanese prior and during the stay)
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No Yes Yes Yes Yes Yes Yes Yes No
Type of Research Project
- Basic science
What is the background of the project?
In mammalian cells, efficient delivery of newly synthesized lysosomal enzymes is carried out by the mannose 6-phosphate receptors, which are intracellularly cycling between the trans-Golgi network, endosomes and plasma membrane.
What is the aim of the project?
In this project, to understand the precise itineraries of this receptors and kinetics of clathrin adaptor molecules that regulate the receptor trafficking, we will tag some of the key proteins with green fluorescent proteins or its variants. They will be expressed in culture cells and observed by video microscopy or immuno-electron microscopy.
What techniques and methods are used?
video microscopy or immuno-electron microscopy.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Students are expected to conduct some of the experiments that will be planned in this laboratory, discuss several scientists in this University, and make a report on the study. Excellent works will be considered for publication in scientific journals.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Journal club with another cell biology laboratory (once a week)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Students are required to study on the basics of the cell biology, for example by reading
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Waguri S; Tomiyama Y; Ikeda H; Hida T; Sakai N; Taniike M; Ebisu S; Uchiyama Y. The luminal domain participates in the endosomal trafficking of the cation-independent mannose 6-phosphate receptor. Exp Cell Res. (2006) Dec 10;312(20):4090-4107
- Waguri S; Dewitte F; Le Borgne R; Rouille Y; Uchiyama Y; Dubremetz JF; Hoflack B. Visualization of TGN to Endosome Trafficking through Fluorescently Labeled MPR and AP-1 in Living Cells. Mol Biol Cell. (2003) 14:142-155