Projects
Name
Neuroendocrine differentiation of prostate cancer and bone metastasis
University
Greece (HelMSIC) - National and Kapodistrian University of Athens, Athens
Domain
Physiology
Departement
75 Micras Asias, Medical School, Goudi, 11527, Athens, Greece
Head
Prof. Michael Koutsilieris
Tutor
Athanasios Armakolas
Languages
English or Greek
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes Yes Yes Yes Yes Yes Yes No Yes Yes Yes Yes
Type of Research Project
- Basic science
What is the background of the project?
Background information:The epithelium of the prostate gland is under hormonal control of androgens. Androgen ablation mostly affects these cells, reducing their number by up to 90%. Clusters of malignant neuroendocrine cells are found in most prostate cancer cells. NE differentiation is the basic character of the prostate, either benign or malignant. These cells hold certain peptide hormones or pro-hormones, which affect the target cells by endocrine, paracrine, autocrine and neuroendocrine transmission in an androgen-independent fashion due to lack of androgen receptor. The extent of neuroendocrine differentiation is associated with progression and prognosis of Prostate cancer and is thought to be one of the most impostant mechanisms for hormone-refractory prostate cancer. Key palyers found from our laboratory to be involved in the neuroendocrine differentiation of prostate cancer are the metabotropic glutamate receptors (mGluRs). mGluRs bind to glutamate, an amino acid that functions as an excitatory neurotransmitter. Different receptors are associated with different functions. In cancer there is evidence from our lab that some MgluRs are associated with self renewal (proliferation) and migration of precursor cells (metastasis).

What is the aim of the project?
To Determine and characterise the function of the MgluRs that are important for the neuroendocrine differentiation in prostate cancer.

Techniques used: Tissue culture of normal prostate cencer cell lines, DNA and RNA extraction, PCR and RT-PCR, Flow cytometry, siRNA

Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)?
There will be preliminary reading (the student will be contacted by the tutor) as well as seminars in English.
What is the aim of the project?
-
What techniques and methods are used?
-
What is the role of the student?
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
What are the tasks expected to be accomplished by the student?
Assist in the Lab Work of the project
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Preferably, theoretical knowledge in molecular biology techniques and laboratory work. There are not any legal limitations in the student
Are there any legal limitations in the student’s involvement
No
Hours
5
Type of students accepted
This project accepts:
- Medical students
Articles
- Anticancer Res. 2007 Sep-Oct;27(5B):3651-60
- European Urology 47 (2005) 147-155
- Int J Urol. 2009 Jan;16(1):37-44