Projects
Name
Mechanism of anti-IFN-gamma autoantibodies disease (Adult Onset Immunodeficiency)
University
Taiwan (FMS-Taiwan) - Chang Gung University, Taipei
Domain
Immunology
Departement
Chang Gung Unviersity, Graduate Institute of Clinical Medical Sciences
Head
Cheng-Lung KU
Tutor
Cheng-Lung KU
Languages
Mandarin, English and French
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Aims: We are interested in studying the molecular feature of anti-IFN- autoAbs and theidiopathic origination of autoAbs. In this proposal, there are four main goals will be achieved: (1) Generation of anti-IFN-? mAbs from autoAbs producing B cells; (2)Characterization of anti-IFN-mAbs concerning the role of somatic mutation and V(D)J usage in binding affinity and neutralizing effect (3) Study the crystal structure of anti-INF-AutoAbs/IFN-complex in collaboration with Dr. Felix A. Rey from Pasteur Institute inParis. (4) Measurement the absolute titer of anti-IFN- from patients to explain the diseaseprogression.
What is the aim of the project?
In this proposal, there are four main goals will be achieved: (1) Generation of anti-IFN-? mAbs from autoAbs producing B cells; (2)Characterization of anti-IFN-mAbs concerning the role of somatic mutation and V(D)J usage in binding affinity and neutralizing effect (3) Study the crystal structure of anti-INF-AutoAbs/IFN-complex in collaboration with Dr. Felix A. Rey from Pasteur Institute inParis. (4) Measurement the absolute titer of anti-IFN- from patients to explain the diseaseprogression.
What techniques and methods are used?
Used techniques: Flow-cytometry, Single cell RT-PCR, Cell culture, Molecular cloning
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
By identifying the epitope, we expect to product a modified IFN-y to restore the IFN-y signaling without autoantibodies interfere. By searching the similar sequence to epitope, we could identify possible microbial antigen as the origin of production of autoantibodies to IFN-y and we will apply more molecular experiment to test this hypothesis of Molecular Mimicry. Anti-IFN-y autoAbs is one unique and threatening disease for our society and is most important prototype of anticytokine diseases. Our studying is very critical for better understand, treatment and prevention of this disease. Dependent the contribution of student work, but the co-authorship on paper or poster on related publication is expected.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
We are particular interesting in “Human Immunology”, which is studying clinical important question by human sample. We are happy to share our enthusiast about studying the human immunology and infectious disease. Participant didn’t need any knowledge about infectious disease, Immunology and Molecular Biology.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Minimum duration: 4 weeks. Maximum duration: 52 weeks.
Approximately 12 hours of work daily.
Are there any legal limitations in the student’s involvement
No
Hours
12
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
Articles
- 2010; Lancet Infect Dis 10 (12): 875-885:{Anticytokine autoantibodies in infectious diseases: pathogenesis and mechanisms}
- 2012; N Engl J Med 367 (8): 725-734:{Adult-onset immunodeficiency in Thailand and Taiwan}
- Lin CH; Chi CY; Shih HP; Ding JY; Lo CC; Wang SY; Kuo CY; Yeh CF; Liu SH; Tu KH; Ho MW; Lin PC; Chen HK; Ho CH; Lee CH; Lai HC and Ku CL. Identification of a major epitope by anti-IFN