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Liver regeneration using autologous bone marrow cells for liver cirrhosis
Japan(IFMSA-Japan) - Yamaguchi University, Yamaguchi
Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine
Taro TAKAMI, M.D., Ph.D. (Associate Professor)
Taro Takami M.D.
Type of Research Project
- Basic science
What is the background of the project?
We previously reported that bone marrow cells (BMCs) infused via a peripheral vein efficiently repopulate the cirrhotic liver. Repopulated BMCs produce collagenases, including matrix metalloproteinase-9 (MMP-9), resulting in reduced liver fibrosis, elevated serum albumin levels, and a significant increase in survival. Based on these data, we began providing “Autologous bone marrow cell infusion (ABMi) therapy” using non-cultured autologous whole BMCs. However, it involves bone marrow (BM) aspiration under general anesthesia. Therefore, we developed a less invasive therapy that uses cultured autologous BM-derived mesenchymal stem cells (BMSCs) aspirated under local anesthesia. We demonstrated that the peripheral infusion of cultured human BMSCs reduced liver fibrosis in immunodeficient cirrhotic mice. Following regulatory approval in Japan, we started using cultured autologous BMSCs for liver regeneration therapy. We are characterizing the mechanisms underlying the effects of the infused cultured BMSCs on liver cirrhosis.
What is the aim of the project?
1) To reveal the underlining mechanisms of liver regeneration after the infusion of cultured bone marrow derived cells in liver cirrhotic animal models. 2) To assess the cell-cell interactions between cultured bone marrow derived cell and hepatic cell in vitro.
What techniques and methods are used?
Cell culture, Western blot, Real-time PCR, ELISA, Immunohisto chemistry, etc
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Please ask us via your NORE
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Please ask us via your NORE
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
We will teach the skill of our experiment and some knowledge of stem cell. Any special knowledge is not required, and there is no legal limitation.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Fujisawa K; et al. Usefulness of Adult Medaka Fish as a Model for the Evaluation of Alcoholic Fatty Liver. Alcohol. 2019;77:147-154
- Nishimura T; et al. Liver regeneration therapy through the hepatic artery-infusion of cultured bone marrow cells in a canine liver fibrosis model. PLoS One. 2019;14(1):e0210588.
- Fujisawa K; et al. Analysis of Metabolomic Changes in Mesenchymal Stem Cells on Treatment with Desferrioxamine as a Hypoxia Mimetic Compared to Hypoxic Conditions. Stem Cells. 2018;36(8):1226-1236
- Takami T; et al. Stem cell therapy in chronic liver disease. Curr Opin Gastroenterol. 2012;28(3):203-208. Review
- Terai S; et al. Improved liver function in liver cirrhosis patients after autologous bone marrow cell infusion therapy Stem Cells 2006 Oct;24(10):2292-8
- Sakaida I et al. Transplantation of bone marrow cells reduces CCl4-induced liver fibrosis in mice. Hepatology. 2004 Dec;40(6):1304-11.
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