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Roles of ion channels on the differentiation of iPS cells
Fukushima Medical University
Department of Cellular and Integrative Physiology, Fukushima Medhical Unievrsity
Prof. Akihiro Hazama, M.D., Ph.D.
Susumu Yoshie PhD
Type of Research Project
- Basic science
What is the background of the project?
iPS cells are induced pluripotent stem cells found in 2006 and many types of tissues have been generated from iPS cells. We are trying to generate airway epithelia from iPS cells. The functions of airway epithelia, such as water secretion and cilia movement, are regulated by ion channels. We have a hypothesis that those ion channels have key roles in the differentiation of epithelia. We want to prove this hypothesis using iPS cells.
What is the aim of the project?
Final Goal of our project is to understand the precise mechanism of epithelial tissue differentiation for the further application of our findings to the regeneration medicine.
What techniques and methods are used?
Standerd techniques of cell culture and molecular biology are used. If applicants are not familier with such techniques, they will learn in our lab.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The students will culture iPS cells under appropriate conditions and observe the differentiation by detecting mature/immature signals using RT-PCR and western blot analysis. The function of diffentiated airway epithelia will be observed the live- imaging using ion sensitive phluorescence dye or electrophysiological mesurements. The students will perform these experiments with our staffs.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Special skills are not required. Even the beginners will join our projects.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
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