Molecular mechanism of the development of organs
University of Tsukuba
Department of Anatomy and Embryology Majors of Medical Sciences
Satoru Takahashi, M.D., Ph.D.
Michito Hamada, Ph.D, Takashi Kudo , Ph.D.
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Basic science
What is the background of the project?
Large Maf transcription factors are important for development of many cells, for example pancreatic endocrine cells, macrophage and podocytes of kidney. We are trying to investigate in vivo functions of Large Maf transcription factors by using genetically modified mice.
What is the aim of the project?
We are trying to investigate in vivo functions of Large Maf transcription factors by using genetically modified mice since mutations of large Maf transcription factor gene are causes of several diseases and functional analysis will contribute to generation of new therapeutic methods.
What techniques and methods are used?
Generation method of genetically modified mice by using CRISPR/Cas9 and histological analysis of mouse and Moleculer Biology techniques.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
We ask students to join our research team and do some part of our research.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Every Monday, we have progress meeting and journal club, which provide you basic knowlleges of reseach.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge of molecular biology, animal experiments, histological analysis, gene engineering.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Hamada M; Nakamura M; Tran MT; Moriguchi T; Hong C; Ohsumi T; Dinh TT; Kusakabe M; Hattori M; Katsumata T; Arai S; Nakashima K; Kudo T; Kuroda E; Wu CH; Kao PH; Sakai M; Shimano H; Miyazaki T; Tontonz P; Takahashi S. MafB promotes atherosclerosis by inhibiting foam-cell apoptosis. Nat Commun. 5; 3147; 2014.
- Morito N; Yoh K; Ojima M; Okamura M; Nakamura M; Hamada M; Yamagata K; Takahashi S. Mafb plays a protective role in diabetic nephropathy through slit- diaphragm proteins; anti-oxidative enzymes and Notch pathways of podocytes. J Am Soc Nephrol. 25; 2546-2557; 2014.