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Development of fluorescent sensor protein to trap transiently aggregating proteins
Fukushima Medical University
Department of Cell Science, 1 Hikarigaoka, Fukushima Medical University School of Medicine, Fukushima, 960-1295, Japan
Ikuo Wada, Ph.D.
Type of Research Project
- Basic science
What is the background of the project?
Folding of newly synthesized proteins in cells accompanies internalization of hydrophobic patches into the interior of the molecules. In the healthy condition, this process is completed with aids of various assisting molecules while the folding intermediates may form transient aggregates. However, when the machinery is deteriorated by aging, the collapse of the sticky domain may be hampered and result in aggregate formation. It depends on the primary structure whether the process is required or not. It is generally difficult to predict from the properly folded structure.
What is the aim of the project?
Basically, development of the robust method to identify the presence of transiently aggregated structure. However, because of the limited time frame, the person is involved in testing the system.
What techniques and methods are used?
General molecular cloning and expression methods. Imaging using conventional microscopes.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Construction of the test probes by recombinant DNA techniques. Usually, we use GIbson Assembly or related methods, this can be done without basic molecular cloning experience. The constructed probes are observed under the confocal microscope and the obtained images are analyzed by several methods. The results are confirmed in live cells using molecular dynamic analysis.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
yes (Journal/data club)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic biochemical lab course skill.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Nature Communications; 6:8858; 2015 doi: 10.1038/ncomms9858
- PLoS ONE 7(5)e37551 2012
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