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The regulation of Chinese herb decoctions on the cytokine or inflammatory mediator gene expression in T cells or epithelial cells
Taiwan (FMS-Taiwan) - Chang Gung University, Chinese Dpt, Taipei
The Microbiology Division of the Institute of Basic Medical Biology
Professor Yi-Ywan Margaret Chen
Prof. Ming-Ling Kuo, PhD.
English or Chinese
Type of Research Project
- Basic science
What is the background of the project?
The secretion of cytokines is the major function of activating T cells. Thus, the regulation on cytokine gene expression becomes the best candidate for the potential target of traditional Chinese medicine (TCM) on T cells. Chinese doctors routinely prescribe one TCM decoction to asthmatic patients. This TCM can inhibit the expression of Th2-type cytokines at the RNA and protein levels. Overall gene regulation of TCM to T cells will be analyzed with cDNA microarray. The effect of TCM on the intracellular calcium concentration and the activity of nuclear binding proteins in activated T cells will also be examined.
What is the aim of the project?
The major goal of this project is to understand more about the effects of traditional Chinese medicine on T cells and immune system.
What techniques and methods are used?
Used Techniques ELISA, RT-PCR, DNA construction, cell culture, flow cytometry, animal asthma model
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
1. Basic knowledge of immunology and molecular biology.
2. Cell culture, RNA extraction, PCR, flow cytometry
Project Duration:4-12 weeks
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
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