Projects
Name
Diagnostic and Treatment of High Risk Neuroblastoma
University
Czech Republic (IFMSA CZ) - Charles University, 2nd Medical Faculty, Prague
Domain
Oncology
Departement
Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine
Head
Prof. Jan Stary , MD., DrSc.
Tutor
Josef Malis, MD., Prof. Tomas Eckschlager, MD., PhD.
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes Yes No No No No No No Yes No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
The clinical course of neuroblastoma is variable and depends on age at diagnosis, staging, histology, and specific genetic abnormalities. Clinical and biologic features of this Neuroblastoma (NBL) is a malignancy of the sympathetic nervous system. It is the most common extracranial solid tumor of infancy that is responsible for more than 1/10 of pediatric cancer deaths in patients younger than 15 years of age. NBL is characterized by extensive clinical heterogenity, which includes everything between spontaneous regression or differentiation and treatment-refractory progression despite intensive multimodal therapy. While the current clinical results show benefit, the majority of children with high-risk NBL are still dying of recurrent or refractory disease, and to date there are no salvage treatment regimens known to be curative The most frequent genetic alteration of high-risk NBL is somatically acquired amplification of the NMYC oncogene and is also a well-established poor prognostic marker for NBL and strongly correlates with higher tumor agressivity and resistance to treatment. However, a number of other genetic changes that are associated with prognosis are known. In addition, many of these genetic changes can serve as therapeutic targets.
What is the aim of the project?
The aim of the project is to analyse the The aim of the project is to analyse the factors of particular genetic abnormalities that enable to choose the appropriate therapy.
What techniques and methods are used?
Molecular cytogenetic methods- FISH, MLPA, CGH array, SNP array
What is the role of the student?
- The student will mainly observe
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- If the project is clinical
- the student will be allowed to work with patients
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The role of the student is working under the supervision of the researcher on the project. The student will participate in laboratory work focused both on the examination of patient samples and experimental research in Laboratory for biology of solid tumors, on the examination of patients and clinical analysis under the supervision of a clinician and laboratory worker. The student will participate also in X-ray seminaries and tumor boards.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
- Internal seminars (weakly) focused on the topic theory and interpretation of experimental results - Seminars and hands-on courses
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
4 years of medical school completed No special skills required, just interest in the laboratory work. No legal limitations, however, work with potentially hazardous substances can be required. For education process will be necessary special clothes: - White medical shoes - White pants - White coat
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
Articles
- Prochazka P; Hrabeta J; Vicha A; Cipro S; Stejskalova E; Musil Z; Vodicka P; Eckschlager T. Changes in MYCN expression in human neuroblastoma cell lines following cisplatin treatment may not be related to MYCN copy numbers. Oncol Rep.2013;29(6):2415-21.
- Stiborová M; Poljaková J; Eckschlager T; Kizek R; Frei E. DNA and histone deacetylases as targets for neuroblastoma treatment. Interdiscip Toxicol. 2010;3(2):47-52
- Eckschlager T; Pilát D; Kodet R; Dahbiová R; Stanková J; Jasinská J; Hrusák O.DNA ploidy in neuroblastoma. Neoplasma. 1996;43(1):23-6
- Maris JM; Hogarty MD; Bagatell R; Cohn SL. Neuroblastoma. Lancet. 2007; 369 (9579):2106-20
- Brisse HJ; McCarville MB; Granata C; Krug KB; Wootton-Gorges SL; Kanegawa K; Giammarile F; Schmidt M; Shulkin BL; Matthay KK; Lewington VJ; Sarnacki S; Hero B; Kaneko M; London WB; Pearson AD; Cohn SL; Monclair T; International Neuroblastoma Risk Group Project. Guidelines for imaging and staging of neuroblastic tumors: consensus report from the International Neuroblastoma Risk Group Project. Radiology. 2011;261(1): 243-57.
- Cohn SL; Pearson AD; London WB; Monclair T; Ambros PF; Brodeur GM; Faldum A; Hero B; Iehara T; Machin D; Mosseri V; Simon T; Garaventa A; Castel V; Matthay KK; INRG Task Force. The International Neuroblastoma Risk Group (INRG) classification system: an INRG Task Force report. J Clin Oncol. 2009;27(2):289-97
- Monclair T; Brodeur GM; Ambros PF; Brisse HJ; Cecchetto G; Holmes K; Kaneko M; London WB; Matthay KK; Nuchtern JG; von Schweinitz D; Simon T; Cohn SL; Pearson AD; INRG Task Force. The International Neuroblastoma Risk Group (INRG) staging system: an INRG Task Force report. J Clin Oncol.
- Matthay KK; George RE; Yu AL. Promising therapeutic targets in neuroblastoma. Clin Cancer Res. 2012;18(10):2740-53.
- Mueller S; Matthay KK. Neuroblastoma: biology and staging. Curr Oncol Rep.2009;11(6): 431-8.