Application of iPS cell technology to Immunology
Hokkaido University
Division of Immunobiology, Institute of Genetic Medicine, Hokkaido University
Ken-ichiro Seino, M.D., Ph.D
Haruka Wada, Ph.D.
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Basic science
What is the background of the project?
Recently iPS cells were established and various technologies regarding the generation of iPS cells and cell reprogramming were developed. We are now investigating whether those technologies are useful to better understand the immune system.
What is the aim of the project?
We have induced iPS cells from one of immune cells, B cells, and, in turn, differentiated iPS cells to immune cells. Several new findings have been found.
What techniques and methods are used?
In this project, students can do many cell cultures, gene transduction, flowcytometry, and PCR, and so on.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
In this project, students can do many cell cultures, gene transduction, flowcytometry, and PCR, and so on.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Besides this research, we also do some additional activities. If you are interested, please feel free to ask us.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge of biochemisitry and molecular biology. One who already had training of basic experimental biochemistry will be better.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Wada H; Kojo S; Kusama C; Okamoto N; Sato Y; Ishizuka B; Seino K. Successful differentiation to T cells; but unsuccessful B-cell generation; from B-cell-derived induced pluripotent stem cells. Int Immunol. 23:65-74; 2011