Projects
Name
Effect of glucocorticoid hormone on T cell development and activation
University
Hungary(HuMSIRC)-University of Pecs,Pecs
Domain
Immunology
Departement
Immunology and Biotechnology
Head
Prof. Peter Nemeth MD, PhD
Tutor
Timea Berki MD, PhD
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No Yes Yes Yes No No Yes Yes Yes Yes Yes No
Type of Research Project
- Basic science
What is the background of the project?
Both endogenous and therapeutically injected glucocorticoid hormones (GC) influence T-cell maturation by inducing apoptosis of immature thymocytes. In addition locally produced GCs were also identified in the thymus. The physiological role of these thymus-derived GCs in thymocyte development and selection is still controversial. Normal T cell development was described in GC receptor (GCR) KO mice. We have demonstrated that among the different thymocyte subpopulations the most GC sensitive DP thymocytes express the lowest GC receptor (GCR) level both at protein and mRNA level. Supporting the mutual antagonism model others describe that GC-signaling can prevent TCR-induced apoptosis and induce positive selection of thymocytes. The survival of developing thymocytes during positive and negative selection steps depends on the TCR signaling. The crosstalk between TCR-signaling and the GC induced signal was described in T-cell hybridoma cells: independently both signals induce apoptosis, yet together they promote T cell survival.
In this project we will study the effects of the antigen (TCR) and GC hormone on the survival and selection of developing thymocytes using a TCR transgenic mouse model. We also investigate the role of glucocorticoid receptor and the signal transducing pathways induced by these two stimuli in T cells.
Tissue culture technique, flow cytometry, Western blots and RT-PCR will be used in this project.
Purpose of the project: Study the role of glucocorticoid hormone and its receptor in T cell maturation and selection.
What is the aim of the project?
Purpose of the project: Study the role of glucocorticoid hormone and its receptor in T cell maturation and selection.
What techniques and methods are used?
Tissue culture technique, flow cytometry, Western blots and RT-PCR will be used in this project
What is the role of the student?
What are the tasks expected to be accomplished by the student?
basic lab work
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
yes
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge on immunology and molecular biology.
Curriculum vitae and reference letter.
Are there any legal limitations in the student’s involvement
No
Hours
5
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
Articles
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