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Glucose sensing-signaling in diabetes mellitus: calcium signaling
Hungary(HuMSIRC)-University of Pecs,Pecs
Clinical Chemistry, Pecs University Faculty of Medicine
Prof. Dr. G
Dr. Attila Miseta, Dr. Peter Csutora
Type of Research Project
- Basic science
What is the background of the project?
Many cell and tissue types (brain, muscle) are critically dependent ont he constant supply of glucose. Glucose sensing is complicated process, which involves various intracellular signalling pathways. This includes the calcium-calmodulin signal system. By genetically knocking out various elements of calcium homeostasis and signalling we established a model system int he yeast Saccharomyces cerevisiae. Intracellular calcium levels are measured in vivo with the aid of an aeqourin expression plasmid. Experiments on mammalian cells are carried out with the aid of fluorescence calcium binding dyes and flow cytometry.
What is the aim of the project?
By genetically knocking out various elements of calcium homeostasis and signalling we established a model system int he yeast Saccharomyces cerevisiae
What techniques and methods are used?
fluorescence calcium binding dyes and flow cytometry.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
basic lab work
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic immunology, physiology and molecular biology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
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